Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- PA5-22265 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RACGAP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse heart. Predicted reactivity: Mouse (84%), Rat (84%), Rhesus Monkey (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Rac1 is deactivated at integrin activation sites through an IQGAP1-filamin-A-RacGAP1 pathway.
Jacquemet G, Morgan MR, Byron A, Humphries JD, Choi CK, Chen CS, Caswell PT, Humphries MJ
Journal of cell science 2013 Sep 15;126(Pt 18):4121-35
Journal of cell science 2013 Sep 15;126(Pt 18):4121-35
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RACGAP1 Polyclonal Antibody (Product # PA5-22265). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: Raji . 7.5% SDS PAGE. RACGAP1 Polyclonal Antibody (Product # PA5-22265) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RACGAP1 Polyclonal Antibody (Product # PA5-22265). Sample (50 µg of whole cell lysate). Lane A: mouse heart . 7.5% SDS PAGE. RACGAP1 Polyclonal Antibody (Product # PA5-22265) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RACGAP1 in paraformaldehyde-fixed A431 cells using a RACGAP1 polyclonal antibody (Product # PA5-22265) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H1299 xenograft , using RACGAP1 (Product # PA5-22265) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using RACGAP1 (Product # PA5-22265) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL