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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX21441 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX21441, RRID:AB_370024
- Product name
- HDAC7 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Western blot analysis of HDAC-7 expresssion in mouse small intestine tissue lysate.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sonicated Chromatin prepared from untreated (UI) or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure with GTX21441 to HDAC7 and the immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are nomalized over inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 10 ul of GTX21441 and 2x10*6cells were used in each ChIP experiment.
