H00051564-M01

antibody from Abnova Corporation

Targeting: HDAC7 DKFZP586J0917, HDAC7A

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00051564-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00051564-M01, RRID:AB_425982

Product name

HDAC7A monoclonal antibody (M01), clone 2B11-1C1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant HDAC7A.

Antigen sequence

MGFCFFNSVAIACRQLQQQSKASKILIVDWDVHHG
NGTQQTFYQDPSVLYISLHRHDDGNFFPGSGAVDE
VGAGSGEGFNVNVAWAGGLDPPMGDPEYLAAFRIV
VMPIAREFSPDLVLVSAGFDAAEGHPAPLGGYHVS
AKCFGYMTQQLMNLAGGAVVLALEGGHDLTAICDA
SEACVAALLGNRVDPLSEEGWKQKPNLNAIRSLEA
VIRVHSKYWGCMQRLASCPDSWVPRVPGADKEEVE
AVTALASLSVGILAEDRPSEQLVEEEEPMNL


Isotype

IgG

Antibody clone number

2B11-1C1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Histone deacetylase 7 and FoxA1 in estrogen-mediated repression of RPRM.

Malik S, Jiang S, Garee JP, Verdin E, Lee AV, O'Malley BW, Zhang M, Belaguli NS, Oesterreich S
Molecular and cellular biology 2010 Jan;30(2):399-412

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

HDAC7A monoclonal antibody (M01), clone 2B11-1C1 Western Blot analysis of HDAC7A expression in K-562 ( Cat # L009V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of HDAC7A expression in transfected 293T cell line by HDAC7A monoclonal antibody (M01), clone 2B11-1C1.Lane 1: HDAC7A transfected lysate(29.6 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged HDAC7 is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol