683504

antibody from BioLegend

Targeting: HDAC7 DKFZP586J0917, HDAC7A

Western blot (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

683504

Provider

BioLegend

Proper citation

BioLegend Cat#683504, RRID:AB_2687138

Product name

Go-ChIP-Grade™ Purified anti-HDAC7

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

11C7C49

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Other assay

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3µg of cross-linked chromatin samples from Jurkat cells with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-HDAC7 Antibody (Clone 11C7C49), B) equal amount of Purified Mouse IgG1, κ Isotype Control Antibody, or C) competitor’s ChIP-grade Purified anti-HDAC7 Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human NucE gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.

Conjugate

Unconjugated