PA5-68836

antibody from Invitrogen Antibodies

Targeting: HDAC7 DKFZP586J0917, HDAC7A

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-68836

Provider

Invitrogen Antibodies

Product name

Anti-HDAC7 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This target displays homology in the following species: Cow: 100%; Dog: 93%; Guinea Pig: 100%; Horse: 93%; Human: 93%; Mouse: 100%; Rat: 100%

Reactivity

Human, Mouse, Rat, Bovine, Canine, Guinea Pig

Host

Rabbit

Isotype

IgG

Vial size

100 µl

Concentration

Lot Dependent

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HDAC7 on rat spleen tissue lysate. The sample was probed with a HDAC7 polyclonal antibody (Product # PA5-68836) using a primary antibody dilution of 1.0 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-HDAC7 Polyclonal Antibody (Product # PA5-68836) and a 102 kDa band corresponding to HDAC7 was observed in Mouse Brain and Rat Brain. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1) and Rat Brain (Lane 2) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). An uncharacterized band at ~130 kDa was also observed in the samples tested..