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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunoprecipitation [1]
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- Product number
- NB100-2146 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2146, RRID:AB_10003275
- Product name
- Rabbit Polyclonal HDAC7 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Predicted molecular weight: 105 kDa. Additional bands may be detected in various extract preparations. Detection of HDAC7 is specifically inhibited with the immunizing peptide.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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- Novus Biologicals (provider)
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- Experimental details
- Immunoprecipitation: HDAC7 Antibody [NB100-2146] - Immunoprecipitation HDAC7 was immunoprecipitated from whole cell extract of human HEK 293T cells expressing HDAC7-FLAG using Rabbit Anti-HDAC7 (KG-17) antibody (NB100-2146). The IP samples were separated on SDS-PAGE and blotted with Rabbit Anti-FLAG antibody. The antibody was developed with Goat anti-Rabbit IgG, Peroxidase conjugate and chemiluminescent substrate. Lane 1: 1.8 ug Antibody immunoprecipitates HDAC7 from a RIPA extract of 180 ug of 293T cells expressing HDAC7-FLAG. Lane 2: negative control (extract without antibody).
