PA5-45906

antibody from Invitrogen Antibodies

Targeting: INPP5A 5PTASE

Provider product page for PA5-45906

Western blot

Other assay

Antibody data

Antibody Data
Antigen structure
References [2]
Comments [0]
Validations
Other assay [3]

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Product number: PA5-45906 - Provider product page
Provider: Invitrogen Antibodies
Product name: INPP5A Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Peptide sequence: VVKLIFRESD NDRKVMLQLE KKLFDYFNQE VFRDNNGTAL LEFDKELSVF Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 93%; Pig: 100%; Rat: 93%
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.5 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

INPP5A protein structure - PA5-45906 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 7 Deficiency of TRIM32 changes levels of INPP5A and IP 3 . (A) Western blot analysis of levels of INPP5A and calbindin in TRIM32 - /- mice and their littermates. (B, C) Relative levels of INPP5A (B) and calbindin (C) were quantified. (D) ELISA assay analysis of level of IP 3 . Data are presented as mean +- SEM. n = 5 mice/genotype. ** p < 0.01, *** p < 0.001.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 4 Cerebellum-enriched INPP5A is downregulated in SCA17 knock-in mice. a Heatmaps of dysregulated genes hierarchically clustered from the cerebellum (CB), striatum (STR), and prefrontal cortex (PFC) of 3-month-old SCA17 KI and age-matched WT mice ( n = 3-4 mice per group). Data were presented as log2 fold change with adjusted P value < 0.05. b Summary of the numbers of differentially expressed genes in the CB, STR, and PFC in KI mice. c Venn diagrams indicating the numbers of downregulated or upregulated genes in the CB, STR, and PFC. d Western blotting of INPP5A levels in different tissues from 3-month-old WT mice. Vinculin was used as a loading control. e Real-time PCR assay of Inpp5a mRNA levels in the CB, STR, and PFC from 3-month-old KI mice. The relative mRNA levels of Inpp5a were obtained by normalizing values to an internal control, GAPDH , and analyzed with Student's t test, t = 5.493, ** P = 0.0054, n = 3 mice per group. f , g Western blotting ( f ) and immunofluorescent staining ( g ) of INPP5A in the cerebellum from 3-month-old WT and KI mice. Scale bar = 100 mum. In f , densitometric ratios of NeuN to beta-actin were normalized to WT and analyzed with Student's t test, t = 6.903, ** P = 0.0023, n = 3 mice per group. Data are represented as mean +- SEM. Source data and full blots are provided as a Source Data file.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Inpp5a Knockdown leads to Purkinje cell degeneration, whereas Inpp5a overexpression reduces Purkinje cell degeneration. a Schematic diagram of AAV-Inpp5a gRNA and AAV-Inpp5a vectors. b Western blotting analysis of INPP5A and calbindin in AAV-control gRNA or AAV-Inpp5a gRNA-injected 3-month-old EIIa-Cas9 transgenic mice cerebellum (left panel). The densitometric ratios of INPP5A to ß-actin and calbindin to ß-actin were normalized to control and analyzed with Student’s t test (right panel). Calbindin, t= 3.095, *P= 0.0213; Inpp5a, t= 5.79, **P= 0.0012, n= 4 mice per group. c Representative immunofluorescence images of AAV-gRNA-injected Cas9 mouse cerebellum without mutant TBP. d Western blotting showing the expression of AAV-Inpp5a or AAV-GFP in the injected KI mouse cerebellum. e Representative immunofluorescence images of AAV-Inpp5a or AAV-GFP-injected 5-month-old KI mouse cerebellum. f Western blotting showing increased calbindin in the cerebellum of SCA17 mice after injection of AAV-Inpp5a. AAV-GFP injection served as control. g Quantification of Purkinje cells (left panel) in e was analyzed with Student’s t test, t= 3.483, *P= 0.0011, n= 4 mice per group, three images were used to count from each mouse. The densitometric ratios of calbindin to vinculin in f were normalized to WT and analyzed with one-way ANOVA followed with Tukey’s multiple comparisons test. F= 12.32, *P< 0.05, **P< 0.005. n= 5 mice per group. h IP3 ELISA assay of cerebellar lysates from 2-mont