Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- BS61295 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- CISH polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to residues in Human CISH.
- Description
- Src homology (SH2) domains are noncatalytic sequences that are conserved among a number of cytoplasmic signaling proteins. These signaling proteins are directly regulated by receptor tyrosine kinases and control the activation of mitogenic signal transduction pathways by such receptors (1-3). For instance, ligand-induced activation of the EGF and PDGF receptors induces dimerization, triggers receptor autophosphorylation on tyrosine residues and results in the binding of a number of cytoplasmic SH2 domain proteins such as PLC-g1 (4,5), Ras GAP (6) and PI 3-kinase p85 (5) to the activated receptors (4,5). The Shc gene encodes proteins with a single SH2 domain but no identifiable catalytic domain. CIS, cytokine-inducible SH2-containing protein, is a 267 amino acid protein with a single 96 amino acid SH2 domain that associates the tyrosine-phosphorylated beta chain of the IL-3 receptor with the tyrosine-phosphorylated Epo receptor (7). CIS was initially described as an immediate early cytokine-responsive gene and appears to be a unique regulator of cytokine signaling (7).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -22°C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Bioworld Technology, Inc (provider)
- Main image
- Experimental details
- Western blot (WB) analysis of CISH pAb at 1:500 dilution Lane1:HELA whole cell lysateLane2:RAW264.7 whole cell lysate Lane3:PC12 whole cell lysate