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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [7]
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- Product number
- PA5-52083 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DAZAP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: STTQETLRSY FSQYGEVVDC VIMKDKTTNQ SRGFGFVKFK DPNCVGTVLA SRPHTLDGRN IDPKPCTPRG MQPERTRPKE GWQKGPRSDN SKSNKIFVGG IPHNCGETEL REYFKKFGVV TEVVMIYDAE KQRPR Highest antigen sequence identity to the following orthologs: Mouse - 99%, Rat - 99%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DAZAP1 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a DAZAP1 Polyclonal Antibody (Product # PA5-52083).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DAZAP1 in Lane 1: Marker (kDa) 230, 110, 82, 49, 32, 26, 18; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a DAZAP1 Polyclonal Antibody (Product # PA5-52083).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of DAZAP1 in human cell line U-251 MG shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using a DAZAP1 Polyclonal Antibody (Product # PA5-52083).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of DAZAP1 in human cell line A-431 using a DAZAP1 Polyclonal Antibody (Product # PA5-52083) shows localization to nucleoplasm and cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DAZAP1 in human skin using DAZAP1 Polyclonal Antibody (Product # PA5-52083).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DAZAP1 in human kidney using DAZAP1 Polyclonal Antibody (Product # PA5-52083).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DAZAP1 in human testis tissue shows strong nuclear positivity in cells of seminiferous ducts. Samples were probed using a DAZAP1 Polyclonal Antibody (Product # PA5-52083).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DAZAP1 in human testis and liver tissues using DAZAP1 Polyclonal Antibody (Product # PA5-52083). Corresponding DAZAP1 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DAZAP1 in human cerebral cortex, kidney, skin and testis using DAZAP1 Polyclonal Antibody (Product # PA5-52083) (A) shows similar protein distribution across tissues to an independent DAZAP1 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DAZAP1 in human liver using DAZAP1 Polyclonal Antibody (Product # PA5-52083) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DAZAP1 in human cerebral cortex using DAZAP1 Polyclonal Antibody (Product # PA5-52083).
