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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [3]
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- Product number
- RQ7423 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- SBDS Antibody / Shwachman-Bodian-Diamond syndrome protein
- Antibody type
- Polyclonal
- Description
- This highly specific SBDS antibody is suitable for use in Western blot/Flow cytometry/Immunofluorescence/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the SBDS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) 293T, 2) HepG2 and 3) HL60 cell lysate with SBDS antibody. Predicted molecular weight ~29 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human 293T, 2) human HepG2, 3) human HL60, 4) human SH-SY5Y, 5) rat brain, 6) rat lung, 7) mouse brain and 8) mouse lung tissue lysate with SBDS antibody. Predicted molecular weight ~29 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human U-2 OS cells with SBDS antibody (green) and Beta Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HL60 cells with SBDS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SBDS antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human Caco-2 cells with SBDS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SBDS antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human HEL cells with SBDS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SBDS antibody.
