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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- MA5-27193 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Fra2 Monoclonal Antibody (OTI7E4)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI7E4
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FOSL2 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with FOSL2 (Product # MA5-27193) monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FOSL2 in 293T, MCF7, PC12, LOVO, HeLa, A549 cells using 35 µg per lane. Samples were probed with FOSL2 (Product # MA5-27193) monoclonal antibody at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Fra2 Monoclonal Antibody (OTI7E4) (Product # MA5-27193) and a ~50 kDa band corresponding to FOSL2 was observed in cell lines tested except in U-2 OS, HEK 293 and K-562. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A-549 (Lane 2), U-2 OS (Lane 3), HEK 293 (Lane 4), K-562 (Lane 5) and Daudi (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
