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- Antibody Data
- Antigen structure
- References [9]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- 14726-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14726-1-AP, RRID:AB_2194787
- Product name
- SPAG5 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated SPAG5 antibody (Cat. #14726-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Fe-doped chrysotile nanotubes containing siRNAs to silence SPAG5 to treat bladder cancer.
DIAPH3 deficiency links microtubules to mitotic errors, defective neurogenesis, and brain dysfunction.
SPAG5-AS1 inhibited autophagy and aggravated apoptosis of podocytes via SPAG5/AKT/mTOR pathway.
p53 suppression is essential for oncogenic SPAG5 upregulation in lung adenocarcinoma.
Kinetochores attached to microtubule-ends are stabilised by Astrin bound PP1 to ensure proper chromosome segregation.
CDC20B is required for deuterosome-mediated centriole production in multiciliated cells.
Phosphorylation of Astrin Regulates Its Kinetochore Function.
Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.
OSBP-related protein 8 (ORP8) interacts with Homo sapiens sperm associated antigen 5 (SPAG5) and mediates oxysterol interference of HepG2 cell cycle.
Liu J, Zhang Y, Zeng H, Wang L, Zhang Q, Wu P, Liu X, Xie H, Xiang W, Liu B, Liu J, Liu X, Xie J, Tang J, Long Z, He L, Xiao M, Xiang L, Cao K
Journal of nanobiotechnology 2021 Jun 23;19(1):189
Journal of nanobiotechnology 2021 Jun 23;19(1):189
DIAPH3 deficiency links microtubules to mitotic errors, defective neurogenesis, and brain dysfunction.
Lau EO, Damiani D, Chehade G, Ruiz-Reig N, Saade R, Jossin Y, Aittaleb M, Schakman O, Tajeddine N, Gailly P, Tissir F
eLife 2021 Apr 26;10
eLife 2021 Apr 26;10
SPAG5-AS1 inhibited autophagy and aggravated apoptosis of podocytes via SPAG5/AKT/mTOR pathway.
Xu J, Deng Y, Wang Y, Sun X, Chen S, Fu G
Cell proliferation 2020 Feb;53(2):e12738
Cell proliferation 2020 Feb;53(2):e12738
p53 suppression is essential for oncogenic SPAG5 upregulation in lung adenocarcinoma.
Wang T, Li K, Song H, Xu D, Liao Y, Jing B, Guo W, Hu M, Kuang Y, Sun B, Ling J, Zhang T, Xu J, Yao F, Deng J
Biochemical and biophysical research communications 2019 May 28;513(2):319-325
Biochemical and biophysical research communications 2019 May 28;513(2):319-325
Kinetochores attached to microtubule-ends are stabilised by Astrin bound PP1 to ensure proper chromosome segregation.
Conti D, Gul P, Islam A, Martín-Durán JM, Pickersgill RW, Draviam VM
eLife 2019 Dec 6;8
eLife 2019 Dec 6;8
CDC20B is required for deuterosome-mediated centriole production in multiciliated cells.
Revinski DR, Zaragosi LE, Boutin C, Ruiz-Garcia S, Deprez M, Thomé V, Rosnet O, Gay AS, Mercey O, Paquet A, Pons N, Ponzio G, Marcet B, Kodjabachian L, Barbry P
Nature communications 2018 Nov 7;9(1):4668
Nature communications 2018 Nov 7;9(1):4668
Phosphorylation of Astrin Regulates Its Kinetochore Function.
Chung HJ, Park JE, Lee NS, Kim H, Jang CY
The Journal of biological chemistry 2016 Aug 19;291(34):17579-92
The Journal of biological chemistry 2016 Aug 19;291(34):17579-92
Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.
Kodani A, Yu TW, Johnson JR, Jayaraman D, Johnson TL, Al-Gazali L, Sztriha L, Partlow JN, Kim H, Krup AL, Dammermann A, Krogan NJ, Walsh CA, Reiter JF
eLife 2015 Aug 22;4
eLife 2015 Aug 22;4
OSBP-related protein 8 (ORP8) interacts with Homo sapiens sperm associated antigen 5 (SPAG5) and mediates oxysterol interference of HepG2 cell cycle.
Zhong W, Zhou Y, Li J, Mysore R, Luo W, Li S, Chang MS, Olkkonen VM, Yan D
Experimental cell research 2014 Apr 1;322(2):227-35
Experimental cell research 2014 Apr 1;322(2):227-35
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 14726-1-AP(SPAG5 antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A431 cells, using SPAG5 antibody 14726-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-SPAG5 (IP:14726-1-AP, 5ug; Detection:14726-1-AP 1:1000) with HeLa cells lysate 3000ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human testis using 14726-1-AP(SPAG5 antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
