AF1136

antibody from R&D Systems

Targeting: RAE1 Gle2, Mnrp41

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

AF1136

Provider

R&D Systems

Product name

Mouse Rae-1 Pan Specific Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse Rae-1 gamma as well as mouse Rae-1 alpha , 1 beta , 1 delta and 1 epsilon in direct ELISAs and Western blots.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

O08604


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Cytotoxicity of Natural Killer Cells Activated Through NKG2D Contributes to the Development of Bronchiolitis Obliterans in a Murine Heterotopic Tracheal Transplant Model.

Kawakami T, Ito K, Matsuda Y, Noda M, Sakurada A, Hoshikawa Y, Okada Y, Ogasawara K
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2017 Sep;17(9):2338-2349

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NK1.1(-) CD4(+) NKG2D(+) T cells suppress DSS-induced colitis in mice through production of TGF-β.

Qian X, Hu C, Han S, Lin Z, Xiao W, Ding Y, Zhang Y, Qian L, Jia X, Zhu G, Gong W
Journal of cellular and molecular medicine 2017 Jul;21(7):1431-1444

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The immunoreceptor NKG2D promotes tumour growth in a model of hepatocellular carcinoma.

Sheppard S, Guedes J, Mroz A, Zavitsanou AM, Kudo H, Rothery SM, Angelopoulos P, Goldin R, Guerra N
Nature communications 2017 Jan 27;8:13930

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NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery.

Spada R, Rojas JM, Pérez-Yagüe S, Mulens V, Cannata-Ortiz P, Bragado R, Barber DF
Journal of leukocyte biology 2015 Mar;97(3):583-98

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CD4(+) NKG2D(+) T cells induce NKG2D down-regulation in natural killer cells in CD86-RAE-1ε transgenic mice.

Lin Z, Wang C, Xia H, Liu W, Xiao W, Qian L, Jia X, Ding Y, Ji M, Gong W
Immunology 2014 Mar;141(3):401-15

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Sustained CTL activation by murine pulmonary epithelial cells promotes the development of COPD-like disease.

Borchers MT, Wesselkamper SC, Curull V, Ramirez-Sarmiento A, Sánchez-Font A, Garcia-Aymerich J, Coronell C, Lloreta J, Agusti AG, Gea J, Howington JA, Reed MF, Starnes SL, Harris NL, Vitucci M, Eppert BL, Motz GT, Fogel K, McGraw DW, Tichelaar JW, Orozco-Levi M
The Journal of clinical investigation 2009 Mar;119(3):636-49

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Rae-1 in Mouse Embryo. Rae-1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Rae-1 Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1136) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to axons of primary sensory neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.