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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB6827 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6827, RRID:AB_1579519
- Product name
- RAE1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of RAE1.
- Antigen sequence
C-NAAEELKPRNKK- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly.
Blower MD, Nachury M, Heald R, Weis K
Cell 2005 Apr 22;121(2):223-34
Cell 2005 Apr 22;121(2):223-34
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- RAE1 polyclonal antibody (Cat # PAB6827) staining (0.03 ug/mL) of HepG2 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- RAE1 polyclonal antibody (Cat # PAB6827)(3.8 ug/mL) staining of paraffin embedded human brain cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
