13347-2-AP

antibody from Proteintech Group

Targeting: MCM6 Mis5

Provider product page for 13347-2-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [34]
Comments [0]
Validations
Western blot [1]
Immunoprecipitation [1]
Immunohistochemistry [1]

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Product number: 13347-2-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#13347-2-AP, RRID:AB_2142537
Product name: MCM6 antibody
Antibody type: Polyclonal
Description: KD/KO validated MCM6 antibody (Cat. #13347-2-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

MCM6 protein structure - 13347-2-AP shown in red.

Submitted references Overexpression of CDT1 inhibits cell cycle progression at S phase by interacting with the mini-chromosome maintenance complex and causes DNA damage.

Tsuyama T, Takayama N, Tanaka R, Arai Y, Yamaguchi Y, Nawata Y, Azuma Y, Tada S
FEBS open bio 2026 Feb;16(2):352-364

Topoisomerase I Inhibition in ETV4-overexpressed Non-Small Cell Lung Cancer Promotes Replication and Transcription Mediated R-Loop Accumulation and DNA Damage.

Zhang J, Wang Y, Cao S, Xie SM, Liu B, Li Y, Hou Y, Meng X, Ruan M, Bu D, Kang J, Li R, Lou L, Wang J, Xing L
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Sep;12(35):e09307

CRL4(DCAF12) regulation of MCMBP ensures optimal licensing of DNA replication.

Yadav AK, Abdirov A, Ondruskova K, Negi S, Jamrichova K, Kolarova K, Dibus N, Krejci J, Polasek-Sedlackova H, Cermak L
Nature communications 2025 Oct 27;16(1):9391

SETD3-mediated histidine methylation of MCM7 regulates DNA replication by facilitating chromatin loading of MCM.

Duan H, Wang S, Shu WJ, Tong Y, Long HZ, Li G, Du HN, Zhao MJ
Science China. Life sciences 2025 Mar;68(3):793-808

The MCM6-c-Myc positive feedback loop mediates bladder cancer progression and cisplatin resistance.

Wang J, Li X, Zhao L, Fan X, Cao J, Wang S, Li K, Wang H, Zhang Y, Wang H, Xu C, Ding L, Che T, Chen S, Yang L
International journal of biological macromolecules 2025 Mar;296:139777

TanGIBLE: A selective probe for evaluating hydrophobicity-exposed defective proteins in live cells.

Iwasa Y, Miyata S, Tomita T, Yokota N, Miyauchi M, Mori R, Matsushita S, Suzuki R, Saeki Y, Kawahara H
The Journal of cell biology 2025 Mar 3;224(3)

Crotonylation of MCM6 enhances chemotherapeutics sensitivity of breast cancer via inducing DNA replication stress.

Song H, Guo Z, Xie K, Liu X, Yang X, Shen R, Wang D
Cell proliferation 2025 Feb;58(2):e13759

Analysis of the prognostic and immune role of MCM6 in pan-cancer and experimental validation in lung adenocarcinoma cells.

Liao M, Li C, Lv J, Yang R, Li J, Wu K, Zhang J, Zhu Q, Shi Y, Zhang X
Scientific reports 2025 Aug 5;15(1):28648

Beyond ribosomal function: RPS6 deficiency suppresses cholangiocarcinoma cell growth by disrupting alternative splicing.

Fu W, Lin Y, Bai M, Yao J, Huang C, Gao L, Mi N, Ma H, Tian L, Yue P, Zhang Y, Zhang J, Ren Y, Ding L, Dai L, Leung JW, Yuan J, Zhang W, Meng W
Acta pharmaceutica Sinica. B 2024 Sep;14(9):3931-3948

RNF125‑mediated ubiquitination of MCM6 regulates the proliferation of human liver hepatocellular carcinoma cells.

Feng X, Song D, Liu X, Liang Y, Jiang P, Wu S, Liu F
Oncology letters 2024 Mar;27(3):105

DiPRO1 distinctly reprograms muscle and mesenchymal cancer cells.

Rich J, Bennaroch M, Notel L, Patalakh P, Alberola J, Issa F, Opolon P, Bawa O, Rondof W, Marchais A, Dessen P, Meurice G, Le-Gall M, Polrot M, Ser-Le Roux K, Mamchaoui K, Droin N, Raslova H, Maire P, Geoerger B, Pirozhkova I
EMBO molecular medicine 2024 Aug;16(8):1840-1885

Systematic analysis of expression and prognostic significance for MCM family in head and neck squamous cell carcinoma.

Sun E, Peng L, Liu Z, Yan Z, Chen M, Zheng J
Histology and histopathology 2024 Apr;39(4):471-482

UBE3A and MCM6 synergistically regulate the proliferation and migration of lung adenocarcinoma cells.

Luo Y, Yang Y, Yang C, Li C, Hu R, Geng W, Kang X, Lin H
FEBS open bio 2023 Sep;13(9):1756-1771

MCM6 is a Poor Prognostic Biomarker and Promotes Progression in Breast Cancer.

Lei Z, Wang P, Jia DQ, Li LL, Wu YP, Yang Y, Pan GQ
Frontiers in bioscience (Landmark edition) 2023 Aug 30;28(8):128

Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma.

Tong Y, Sun M, Chen L, Wang Y, Li Y, Li L, Zhang X, Cai Y, Qie J, Pang Y, Xu Z, Zhao J, Zhang X, Liu Y, Tian S, Qin Z, Feng J, Zhang F, Zhu J, Xu Y, Lou W, Ji Y, Zhao J, He F, Hou Y, Ding C
Journal of hematology & oncology 2022 Nov 25;15(1):168

Cadherin-16 inhibits thyroid carcinoma cell proliferation and invasion.

Yang X, Li Y, Liu G, Zha W, Liu Y
Oncology letters 2022 May;23(5):145

Small-molecule inhibitors that disrupt the MTDH-SND1 complex suppress breast cancer progression and metastasis.

Shen M, Wei Y, Kim H, Wan L, Jiang YZ, Hang X, Raba M, Remiszewski S, Rowicki M, Wu CG, Wu S, Zhang L, Lu X, Yuan M, Smith HA, Zheng A, Bertino J, Jin JF, Xing Y, Shao ZM, Kang Y
Nature cancer 2022 Jan;3(1):43-59

ACTL6A deficiency induces apoptosis through impairing DNA replication and inhibiting the ATR-Chk1 signaling in glioblastoma cells.

Hu X, Pei D, Ci M, Zhang G, Li B, Wang J, Shen Y, Zhai X, Liang P, Cui H
Biochemical and biophysical research communications 2022 Apr 9;599:148-155

MCMBP promotes the assembly of the MCM2-7 hetero-hexamer to ensure robust DNA replication in human cells.

Saito Y, Santosa V, Ishiguro KI, Kanemaki MT
eLife 2022 Apr 19;11

The influence of phenolic environmental estrogen on the transcriptome of uterine leiomyoma cells: A whole transcriptome profiling-based analysis.

Li Z, Yin H, Shen Y, Ren M, Xu X
Ecotoxicology and environmental safety 2021 Mar 15;211:111945

Plasma membrane calcium ATPase 1 regulates human umbilical vein endothelial cell angiogenesis and viability.

Njegic A, Swiderska A, Marris C, Armesilla AL, Cartwright EJ
Journal of molecular and cellular cardiology 2021 Jul;156:79-81

Highly expressed of SERPINA3 indicated poor prognosis and involved in immune suppression in glioma.

Yuan Q, Wang SQ, Zhang GT, He J, Liu ZD, Wang MR, Cai HQ, Wan JH
Immunity, inflammation and disease 2021 Dec;9(4):1618-1630

METTL3 Promotes the Progression of Gastric Cancer via Targeting the MYC Pathway.

Yang DD, Chen ZH, Yu K, Lu JH, Wu QN, Wang Y, Ju HQ, Xu RH, Liu ZX, Zeng ZL
Frontiers in oncology 2020;10:115

Minichromosome maintenance 6 complex component identified by bioinformatics analysis and experimental validation in esophageal squamous cell carcinoma.

Li X, Ren Z, Xiong C, Geng J, Li Y, Liu C, Ren C, Liu H
Oncology reports 2020 Sep;44(3):987-1002

RNF8 promotes efficient DSB repair by inhibiting the pro-apoptotic activity of p53 through regulating the function of Tip60.

Chen H, Shan J, Liu J, Feng Y, Ke Y, Qi W, Liu W, Zeng X
Cell proliferation 2020 Mar;53(3):e12780

CtIP promotes G2/M arrest in etoposide-treated HCT116 cells in a p53-independent manner.

Chen H, Shan J, Chen D, Wang R, Qi W, Wang H, Ke Y, Liu W, Zeng X
Journal of cellular physiology 2019 Jul;234(7):11871-11881

Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.

Song H, Guo T, Zhao Z, Wei Y, Luo H, Weng W, Zhang R, Zhong M, Chen C, Su J, Shen W
Biomaterials 2018 Sep;178:23-35

MCM6 promotes metastasis of hepatocellular carcinoma via MEK/ERK pathway and serves as a novel serum biomarker for early recurrence.

Liu M, Hu Q, Tu M, Wang X, Yang Z, Yang G, Luo R
Journal of experimental & clinical cancer research : CR 2018 Jan 22;37(1):10

Overexpression of MCM6 predicts poor survival in patients with glioma.

Cai HQ, Cheng ZJ, Zhang HP, Wang PF, Zhang Y, Hao JJ, Wang MR, Wan JH
Human pathology 2018 Aug;78:182-187

MCMs expression in lung cancer: implication of prognostic significance.

Liu YZ, Wang BS, Jiang YY, Cao J, Hao JJ, Zhang Y, Xu X, Cai Y, Wang MR
Journal of Cancer 2017;8(18):3641-3647

RACK1 promotes lung cancer cell growth via an MCM7/RACK1/ Akt signaling complex.

Fei L, Ma Y, Zhang M, Liu X, Luo Y, Wang C, Zhang H, Zhang W, Han Y
Oncotarget 2017 Jun 20;8(25):40501-40513

Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis.

Ma Y, Fei L, Zhang M, Zhang W, Liu X, Wang C, Luo Y, Zhang H, Han Y
Oncotarget 2017 Dec 1;8(62):104813-104830

Docetaxel-loaded solid lipid nanoparticles suppress breast cancer cells growth with reduced myelosuppression toxicity.

Yuan Q, Han J, Cong W, Ge Y, Ma D, Dai Z, Li Y, Bi X
International journal of nanomedicine 2014;9:4829-46

A panel of protein markers for the early detection of lung cancer with bronchial brushing specimens.

Liu YZ, Jiang YY, Wang BS, Hao JJ, Shang L, Zhang TT, Cao J, Xu X, Zhan QM, Wang MR
Cancer cytopathology 2014 Nov;122(11):833-41

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse testis tissue were subjected to SDS PAGE followed by western blot with 13347-2-AP(MCM6 antibody) at dilution of 1:500
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-MCM6 (IP:13347-2-AP, 3ug; Detection:13347-2-AP 1:1000) with HeLa cells lysate 3800ug.
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The MCM6 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human MCM6. This antibody recognizes human,mouse,rat antigen. The MCM6 antibody has been validated for the following applications: ELISA, WB, IP, IHC analysis.
Appendix: 13347-2-AP.pdf

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