PA5-35922

antibody from Invitrogen Antibodies

Targeting: MCM6 Mis5

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-35922

Provider

Invitrogen Antibodies

Product name

MCM6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2. Predicted reactivity: Mouse (95%), Rat (96%), Rhesus Monkey (100%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCM6 Polyclonal Antibody detects MCM6 protein at nucleus by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCM6 protein stained by MCM6 Polyclonal Antibody (Product # PA5-35922) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of MCM6 was performed using 70% confluent PC-3 log phase cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MCM6 Polyclonal Antibody (Product # PA5-35922) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents cells with no primary antibody to assess background. The images were captured at 60X magnification..

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cross-linked ChIP was performed with HeLa chromatin extract and 5 µg of either control rabbit IgG or MCM6 Polyclonal Antibody (Product # PA5-35922). The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Enrichment of endogenous MCM6 protein at specific gene loci using Anti-MCM6 Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-MCM6 Rabbit Polyclonal Antibody (Product # PA5-35922, 5 µg) on sheared chromatin from 2 million Hela cells using the MAGnify ChIP System kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over cMYC and SAT 2. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCM6 Polyclonal Antibody immunoprecipitates MCM6 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 µg whole cell lysate/extract of MCM6 protein expressing 293T cells B : Control with 3 µg of pre-immune rabbit IgG C : Immunoprecipitation of MCM6 by 3 µg of MCM6 Polyclonal Antibody (Product # PA5-35922) 7.5% SDS-PAGE The immunoprecipitated MCM6 protein was detected by MCM6 Polyclonal Antibody (Product # PA5-35922) diluted at 1 : 500. Anti-rabbit IgG (HRP) was used as a secondary reagent.