H00004175-B01P

antibody from Abnova Corporation

Targeting: MCM6 Mis5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004175-B01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00004175-B01P, RRID:AB_10718742

Product name

MCM6 purified MaxPab mouse polyclonal antibody (B01P)

Antibody type

Polyclonal

Description

Mouse polyclonal antibody raised against a full-length human MCM6 protein.

Antigen sequence

MDLAAAAEPGAGSQHLEVRDEVAEKCQKLFLDFLE
EFQSSDGEIKYLQLAEELIRPERNTLVVSFVDLEQ
FNQQLSTTIQEEFYRVYPYLCRALKTFVKDRKEIP
LAKDFYVAFQDLPTRHKIRELTSSRIGLLTRISGQ
VVRTHPVHPELVSGTFLCLDCQTVIRDVEQQFKYT
QPNICRNPVCANRRRFLLDTNKSRFVDFQKVRIQE
TQAELPRGSIPRSLEVILRAEAVESAQAGDKCDFT
GTLIVVPDVSKLSTPGARAETNSRVSGVDGYETEG
IRGLRALGVRDLSYRLVFLACCVAPTNPRFGGKEL
RDEEQTAESIKNQMTVKEWEKVFEMSQDKNLYHNL
CTSLFPTIHGNDEVKRGVLLMLFGGVPKTTGEGTS
LRGDINVCIVGDPSTAKSQFLKHVEEFSPRAVYTS
GKASSAAGLTAAVVRDEESHEFVIEAGALMLADNG
VCCIDEFDKMDVRDQVAIHEAMEQQTISITKAGVK
ATLNARTSILAAANPISGHYDRSKSLKQNINLSAP
IMSRFDLFFILVDECNEVTDYAIARRIVDLHSRIE
ESIDRVYSLDDIRRYLLFARQFKPKISKESEDFIV
EQYKHLRQRDGSGVTKSSWRITVRQLESMIRLSEA
MARMHCCDEVQPKHVKEAFRLLNKSIIRVETPDVN
LDQEEEIQMEVDEGAGGINGHADSPAPVNGINGYN
EDINQESAPKASLRLGFSEYCRISNLIVLHLRKVE
EEEDESALKRSELVNWYLKEIESEIDSEEELINKK
RIIEKVIHRLTHYDHVLIELTQAGLKGSTEGSESY
EEDPYLVVNPNYLLED


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of MCM6 expression in transfected 293T cell line (H00004175-T01) by MCM6 MaxPab polyclonal antibody.Lane 1: MCM6 transfected lysate(90.31 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

MCM6 MaxPab polyclonal antibody. Western Blot analysis of MCM6 expression in human placenta.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to MCM6 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of purified MaxPab antibody to MCM6 on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between CDC6 and MCM6. Mahlavu cells were stained with anti-CDC6 rabbit purified polyclonal 1:1200 and anti-MCM6 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)