15821-1-AP

antibody from Proteintech Group

Targeting: PKM OIP3, PK3, PKM2, THBP1

Provider product page for 15821-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [78]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]
Immunohistochemistry [2]

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Product number: 15821-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#15821-1-AP, RRID:AB_2163820
Product name: PKM1-specific antibody
Antibody type: Polyclonal
Description: KD/KO validated PKM1-specific antibody (Cat. #15821-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IHC, IP, WB, ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

PKM protein structure - 15821-1-AP shown in red.

Submitted references Icariin ameliorates glycolytic dysfunction in Alzheimer's disease models by activating the Wnt/β-catenin signaling pathway.

Liu J, Wei AH, Liu TT, Ji XH, Zhang Y, Yan F, Chen MX, Hu JB, Zhou SY, Shi JS, Jin H, Jin F
The FEBS journal 2024 May;291(10):2221-2241

Serine/Arginine-Rich Splicing Factor 7 Knockdown Inhibits Aerobic Glycolysis and Growth in HepG2 Cells by Regulating PKM2 Expression.

Shi W, Yao X, Cao X, Fu Y, Wang Y
Current issues in molecular biology 2024 May 20;46(5):5023-5036

USP46 enhances tamoxifen resistance in breast cancer cells by stabilizing PTBP1 to facilitate glycolysis.

Gao S, Wang Y, Xu Y, Liu L, Liu S
Biochimica et biophysica acta. Molecular basis of disease 2024 Mar;1870(3):167011

GL-V9 inhibits the activation of AR-AKT-HK2 signaling networks and induces prostate cancer cell apoptosis through mitochondria-mediated mechanism.

Wang R, Min Q, Guo Y, Zhou Y, Zhang X, Wang D, Gao Y, Wei L
iScience 2024 Mar 15;27(3):109246

Pyruvate kinase M in germ cells is essential for sperm motility and male fertility but not spermatogenesis.

Qian GQ, Wang XC, Zhang X, Shen B, Liu Q
Asian journal of andrology 2024 Mar 1;26(2):212-219

Integrating network pharmacology and bioinformatics to explore and experimentally verify the regulatory effect of Buyang Huanwu decoction on glycolysis and angiogenesis after cerebral infarction.

Tian F, Yi J, Liu Y, Chen B, Wang X, Ouyang Y, Liu J, Tang Y, Long H, Liu B
Journal of ethnopharmacology 2024 Jan 30;319(Pt 3):117218

Excessive SOX8 reprograms energy and iron metabolism to prime hepatocellular carcinoma for ferroptosis.

Yang X, Gu C, Cai J, Li F, He X, Luo L, Xiao W, Hu B, Hu J, Qian H, Ren S, Zhang L, Zhu X, Yang L, Yang J, Yang Z, Zheng Y, Huang X, Wang Z
Redox biology 2024 Feb;69:103002

LncRNA CCAT1 facilitates the progression of gastric cancer via PTBP1-mediated glycolysis enhancement.

Zhang C, Wang H, Liu Q, Dai S, Tian G, Wei X, Li X, Zhao L, Shan B
Journal of experimental & clinical cancer research : CR 2023 Sep 23;42(1):246

USP35 promotes hepatocellular carcinoma progression by protecting PKM2 from ubiquitination‑mediated degradation.

Lv T, Zhang B, Jiang C, Zeng Q, Yang J, Zhou Y
International journal of oncology 2023 Oct;63(4)

The Complex Effects of PKM2 and PKM2:IP(3)R Disruption on Intracellular Ca(2+) Handling and Cellular Functions.

Lemos FO, de Ridder I, Bootman MD, Bultynck G, Parys JB
Cells 2023 Oct 26;12(21)

Phosphorylated YBX2 is stabilized to promote glycolysis in brown adipocytes.

Zhao Q, Yu C, Xu X, Jin W, Zhang Z, Huang H, Gao Y, Pan D
iScience 2023 Oct 20;26(10):108091

Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence.

Wu Y, Tang L, Huang H, Yu Q, Hu B, Wang G, Ge F, Yin T, Li S, Yu X
Nature communications 2023 Mar 10;14(1):1323

PKM2-mediated neuronal hyperglycolysis enhances the risk of Parkinson's disease in diabetic rats.

Zhao Y, Wang Y, Wu Y, Tao C, Xu R, Chen Y, Qian L, Xu T, Lian X
Journal of pharmaceutical analysis 2023 Feb;13(2):187-200

Microglia PKM2 Mediates Neuroinflammation and Neuron Loss in Mice Epilepsy through the Astrocyte C3-Neuron C3R Signaling Pathway.

Li X, Zhou R, Peng H, Peng J, Li Q, Mei M
Brain sciences 2023 Feb 3;13(2)

Lactylation of PKM2 Suppresses Inflammatory Metabolic Adaptation in Pro-inflammatory Macrophages.

Wang J, Yang P, Yu T, Gao M, Liu D, Zhang J, Lu C, Chen X, Zhang X, Liu Y
International journal of biological sciences 2022;18(16):6210-6225

MicroRNA and Metabolic Profiling of a Primary Ovarian Neuroendocrine Carcinoma Pulmonary-Type Reveals a High Degree of Similarity with Small Cell Lung Cancer.

Miglietta S, Girolimetti G, Marchio L, Sollazzo M, Laprovitera N, Coluccelli S, De Biase D, De Leo A, Santini D, Kurelac I, Iommarini L, Ghelli A, Campana D, Ferracin M, Perrone AM, Gasparre G, Porcelli AM
Non-coding RNA 2022 Sep 25;8(5)

Myeloid-Specific Pyruvate-Kinase-Type-M2-Deficient Mice Are Resistant to Acute Lung Injury.

Sun X, Shi F, Wang W, Wu Y, Qu S, Li J, Liang H, Zen K
Biomedicines 2022 May 21;10(5)

Celastrol mitigates inflammation in sepsis by inhibiting the PKM2-dependent Warburg effect.

Luo P, Zhang Q, Zhong TY, Chen JY, Zhang JZ, Tian Y, Zheng LH, Yang F, Dai LY, Zou C, Li ZJ, Liu JH, Wang JG
Military Medical Research 2022 May 20;9(1):22

Kaempferol Can Reverse the 5-Fu Resistance of Colorectal Cancer Cells by Inhibiting PKM2-Mediated Glycolysis.

Wu H, Du J, Li C, Li H, Guo H, Li Z
International journal of molecular sciences 2022 Mar 24;23(7)

ASO-Based PKM Splice-Switching Therapy Inhibits Hepatocellular Carcinoma Growth.

Ma WK, Voss DM, Scharner J, Costa ASH, Lin KT, Jeon HY, Wilkinson JE, Jackson M, Rigo F, Bennett CF, Krainer AR
Cancer research 2022 Mar 1;82(5):900-915

Pyruvate Kinase M2 Protects Heart from Pressure Overload-Induced Heart Failure by Phosphorylating RAC1.

Ni L, Lin B, Hu L, Zhang R, Fu F, Shen M, Yang J, Shi D
Journal of the American Heart Association 2022 Jun 7;11(11):e024854

Spatiotemporal feedforward between PKM2 tetramers and mTORC1 prompts mTORC1 activation.

Xia Y, Wang S, Song C, Luo RY
Physical biology 2022 Jun 13;19(4)

Paeoniflorin ameliorates airway inflammation and immune response in ovalbumin induced asthmatic mice: From oxidative stress to autophagy.

Han X, Hu S, Yang Q, Sang X, Tang D, Cao G
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Feb;96:153835

Inhibiting pyruvate kinase muscle isoform 2 regresses group 2 pulmonary hypertension induced by supra-coronary aortic banding.

Xiong PY, Motamed M, Chen KH, Dasgupta A, Potus F, Tian L, Martin A, Mewburn J, Jones O, Thébaud A, Archer SL
Acta physiologica (Oxford, England) 2022 Feb;234(2):e13764

GM3 synthase deficiency increases brain glucose metabolism in mice.

Bharathi SS, Zhang BB, Paul E, Zhang Y, Schmidt AV, Fowler B, Wu Y, Tiemeyer M, Inamori KI, Inokuchi JI, Goetzman ES
Molecular genetics and metabolism 2022 Dec;137(4):342-348

Capsaicin ameliorates inflammation in a TRPV1-independent mechanism by inhibiting PKM2-LDHA-mediated Warburg effect in sepsis.

Zhang Q, Luo P, Xia F, Tang H, Chen J, Zhang J, Liu D, Zhu Y, Liu Y, Gu L, Zheng L, Li Z, Yang F, Dai L, Liao F, Xu C, Wang J
Cell chemical biology 2022 Aug 18;29(8):1248-1259.e6

A non-canonical role for pyruvate kinase M2 as a functional modulator of Ca(2+) signalling through IP(3) receptors.

Lavik AR, McColl KS, Lemos FO, Kerkhofs M, Zhong F, Harr M, Schlatzer D, Hamada K, Mikoshiba K, Crea F, Bultynck G, Bootman MD, Parys JB, Distelhorst CW
Biochimica et biophysica acta. Molecular cell research 2022 Apr;1869(4):119206

PT109, a novel multi-kinase inhibitor suppresses glioblastoma multiforme through cell reprogramming: Involvement of PTBP1/PKM1/2 pathway.

Yang Y, Tu Y, Lu J, Chen Q, Zhu Z, Peng W, Zhu W, Wen S, Zhang J, Yin W, Pi R
European journal of pharmacology 2022 Apr 5;920:174837

Myostatin Mutation Promotes Glycolysis by Increasing Phosphorylation of Phosphofructokinase via Activation of PDE5A-cGMP-PKG in Cattle Heart.

Gu M, Zhou X, Zhu L, Gao Y, Gao L, Bai C, Yang L, Li G
Frontiers in cell and developmental biology 2021;9:774185

Dihydropyrimidinase Like 2 Promotes Bladder Cancer Progression via Pyruvate Kinase M2-Induced Aerobic Glycolysis and Epithelial-Mesenchymal Transition.

Zou J, Huang R, Chen Y, Huang X, Li H, Liang P, Chen S
Frontiers in cell and developmental biology 2021;9:641432

Targeting HLA-F suppresses the proliferation of glioma cells via a reduction in hexokinase 2-dependent glycolysis.

Chen X, Sun N, Li R, Sang X, Li X, Zhao J, Han J, Yang J, Ikezoe T
International journal of biological sciences 2021;17(5):1263-1276

Kaempferol Reverses Aerobic Glycolysis via miR-339-5p-Mediated PKM Alternative Splicing in Colon Cancer Cells.

Wu H, Cui M, Li C, Li H, Dai Y, Cui K, Li Z
Journal of agricultural and food chemistry 2021 Mar 17;69(10):3060-3068

Downregulation of GLUT3 impairs STYK1/NOK-mediated metabolic reprogramming and proliferation in NIH-3T3 cells.

Shi W, Fu Y, Wang Y
Oncology letters 2021 Jul;22(1):527

Transcriptional dysregulation of TRIM29 promotes colorectal cancer carcinogenesis via pyruvate kinase-mediated glucose metabolism.

Han J, Zhao Z, Zhang N, Yang Y, Ma L, Feng L, Zhang X, Zuo J, Fan Z, Wang Y, Song Y, Wang G
Aging 2021 Jan 20;13(4):5034-5054

Single Mutation in the NFU1 Gene Metabolically Reprograms Pulmonary Artery Smooth Muscle Cells.

James J, Zemskova M, Eccles CA, Varghese MV, Niihori M, Barker NK, Luo M, Mandarino LJ, Langlais PR, Rafikova O, Rafikov R
Arteriosclerosis, thrombosis, and vascular biology 2021 Feb;41(2):734-754

The HIF-1α antisense long non-coding RNA drives a positive feedback loop of HIF-1α mediated transactivation and glycolysis.

Zheng F, Chen J, Zhang X, Wang Z, Chen J, Lin X, Huang H, Fu W, Liang J, Wu W, Li B, Yao H, Hu H, Song E
Nature communications 2021 Feb 26;12(1):1341

Deubiquitinase PSMD14 promotes ovarian cancer progression by decreasing enzymatic activity of PKM2.

Sun T, Liu Z, Bi F, Yang Q
Molecular oncology 2021 Dec;15(12):3639-3658

Asparagine reinforces mTORC1 signaling to boost thermogenesis and glycolysis in adipose tissues.

Xu Y, Shi T, Cui X, Yan L, Wang Q, Xu X, Zhao Q, Xu X, Tang QQ, Tang H, Pan D
The EMBO journal 2021 Dec 15;40(24):e108069

Differential localization patterns of pyruvate kinase isoforms in murine naïve, formative, and primed pluripotent states.

Dierolf JG, Watson AJ, Betts DH
Experimental cell research 2021 Aug 15;405(2):112714

Glucose-6-phosphate dehydrogenase deficiency contributes to metabolic abnormality and pulmonary hypertension.

Varghese MV, James J, Rafikova O, Rafikov R
American journal of physiology. Lung cellular and molecular physiology 2021 Apr 1;320(4):L508-L521

A glycolysis-based 4-mRNA signature correlates with the prognosis and cell cycle process in patients with bladder cancer.

Zhang C, Gou X, He W, Yang H, Yin H
Cancer cell international 2020;20:177

JAK2(V617F) Mutation Promoted IL-6 Production and Glycolysis via Mediating PKM1 Stabilization in Macrophages.

Li R, Sun N, Chen X, Li X, Zhao J, Cheng W, Hua H, Fukatsu M, Mori H, Takahashi H, Ohkawara H, Fukami M, Okamoto M, Hamazaki Y, Zheng K, Yang J, Ikezoe T
Frontiers in immunology 2020;11:589048

Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.

Wang C, Li Y, Yan S, Wang H, Shao X, Xiao M, Yang B, Qin G, Kong R, Chen R, Zhang N
Nature communications 2020 Jun 22;11(1):3162

Overexpression of Pyruvate Kinase M2 in Tumor Tissues Is Associated with Poor Prognosis in Patients with Hepatocellular Carcinoma.

Zhao R, Li L, Yang J, Niu Q, Wang H, Qin X, Zhu N, Shi A
Pathology oncology research : POR 2020 Apr;26(2):853-860

Intestinal epithelial PKM2 serves as a safeguard against experimental colitis via activating β-catenin signaling.

Sun X, Yao L, Liang H, Wang D, He Y, Wei Y, Ye L, Wang K, Li L, Chen J, Zhang CY, Xu G, Wang F, Zen K
Mucosal immunology 2019 Nov;12(6):1280-1290

Nicotinamide Riboside Augments the Aged Human Skeletal Muscle NAD(+) Metabolome and Induces Transcriptomic and Anti-inflammatory Signatures.

Elhassan YS, Kluckova K, Fletcher RS, Schmidt MS, Garten A, Doig CL, Cartwright DM, Oakey L, Burley CV, Jenkinson N, Wilson M, Lucas SJE, Akerman I, Seabright A, Lai YC, Tennant DA, Nightingale P, Wallis GA, Manolopoulos KN, Brenner C, Philp A, Lavery GG
Cell reports 2019 Aug 13;28(7):1717-1728.e6

Trichostatin a Protects Dendritic Cells Against Oxygen-Glucose Deprivation via the SRSF3/PKM2/Glycolytic Pathway.

Jiang H, Zhang S, Song T, Guan X, Zhang R, Chen X
Frontiers in pharmacology 2018;9:612

Biventricular Increases in Mitochondrial Fission Mediator (MiD51) and Proglycolytic Pyruvate Kinase (PKM2) Isoform in Experimental Group 2 Pulmonary Hypertension-Novel Mitochondrial Abnormalities.

Xiong PY, Tian L, Dunham-Snary KJ, Chen KH, Mewburn JD, Neuber-Hess M, Martin A, Dasgupta A, Potus F, Archer SL
Frontiers in cardiovascular medicine 2018;5:195

Proteomic analysis of rat serum revealed the effects of chronic sleep deprivation on metabolic, cardiovascular and nervous system.

Ma B, Chen J, Mu Y, Xue B, Zhao A, Wang D, Chang D, Pan Y, Liu J
PloS one 2018;13(9):e0199237

NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.

Huang D, Chen J, Yang L, Ouyang Q, Li J, Lao L, Zhao J, Liu J, Lu Y, Xing Y, Chen F, Su F, Yao H, Liu Q, Su S, Song E
Nature immunology 2018 Oct;19(10):1112-1125

HIF1 mediates a switch in pyruvate kinase isoforms after myocardial infarction.

Williams AL, Khadka V, Tang M, Avelar A, Schunke KJ, Menor M, Shohet RV
Physiological genomics 2018 Jul 1;50(7):479-494

Editing activity for eliminating mischarged tRNAs is essential in mammalian mitochondria.

Hilander T, Zhou XL, Konovalova S, Zhang FP, Euro L, Chilov D, Poutanen M, Chihade J, Wang ED, Tyynismaa H
Nucleic acids research 2018 Jan 25;46(2):849-860

Pyruvate kinase M1 interacts with A-Raf and inhibits endoplasmic reticulum stress-induced apoptosis by activating MEK1/ERK pathway in mouse insulinoma cells.

Horiuchi Y, Nakatsu D, Kano F, Murata M
Cellular signalling 2017 Oct;38:212-222

Pkm2 can enhance pluripotency in ESCs and promote somatic cell reprogramming to iPSCs.

Qin S, Yang D, Chen K, Li H, Zhang L, Li Y, Le R, Li X, Gao S, Kang L
Oncotarget 2017 Oct 13;8(48):84276-84284

RGD-modified oncolytic adenovirus-harboring shPKM2 exhibits a potent cytotoxic effect in pancreatic cancer via autophagy inhibition and apoptosis promotion.

Xu Y, Chu L, Yuan S, Yang Y, Yang Y, Xu B, Zhang K, Liu XY, Wang R, Fang L, Chen Z, Liang Z
Cell death & disease 2017 Jun 1;8(6):e2835

Oroxylin A activates PKM1/HNF4 alpha to induce hepatoma differentiation and block cancer progression.

Wei L, Dai Y, Zhou Y, He Z, Yao J, Zhao L, Guo Q, Yang L
Cell death & disease 2017 Jul 20;8(7):e2944

Pyruvate kinase type M2 promotes tumour cell exosome release via phosphorylating synaptosome-associated protein 23.

Wei Y, Wang D, Jin F, Bian Z, Li L, Liang H, Li M, Shi L, Pan C, Zhu D, Chen X, Hu G, Liu Y, Zhang CY, Zen K
Nature communications 2017 Jan 9;8:14041

Up-regulation of PKM2 promote malignancy and related to adverse prognostic risk factor in human gallbladder cancer.

Lu W, Cao Y, Zhang Y, Li S, Gao J, Wang XA, Mu J, Hu YP, Jiang L, Dong P, Gong W, Liu Y
Scientific reports 2016 Jun 10;6:26351

PKM2 uses control of HuR localization to regulate p27 and cell cycle progression in human glioblastoma cells.

Mukherjee J, Ohba S, See WL, Phillips JJ, Molinaro AM, Pieper RO
International journal of cancer 2016 Jul 1;139(1):99-111

M-Type Pyruvate Kinase Isoforms and Lactate Dehydrogenase A in the Mammalian Retina: Metabolic Implications.

Casson RJ, Wood JP, Han G, Kittipassorn T, Peet DJ, Chidlow G
Investigative ophthalmology & visual science 2016 Jan 1;57(1):66-80

Alterations in glucose metabolism proteins responsible for the Warburg effect in esophageal squamous cell carcinoma.

de Andrade Barreto E, de Souza Santos PT, Bergmann A, de Oliveira IM, Wernersbach Pinto L, Blanco T, Rossini A, Pinto Kruel CD, Mattos Albano R, Ribeiro Pinto LF
Experimental and molecular pathology 2016 Aug;101(1):66-73

ROS-mediated glucose metabolic reprogram induces insulin resistance in type 2 diabetes.

Dong K, Ni H, Wu M, Tang Z, Halim M, Shi D
Biochemical and biophysical research communications 2016 Aug 5;476(4):204-211

Embryonic MicroRNA-369 Controls Metabolic Splicing Factors and Urges Cellular Reprograming.

Konno M, Koseki J, Kawamoto K, Nishida N, Matsui H, Dewi DL, Ozaki M, Noguchi Y, Mimori K, Gotoh N, Tanuma N, Shima H, Doki Y, Mori M, Ishii H
PloS one 2015;10(7):e0132789

L-cysteine reversibly inhibits glucose-induced biphasic insulin secretion and ATP production by inactivating PKM2.

Nakatsu D, Horiuchi Y, Kano F, Noguchi Y, Sugawara T, Takamoto I, Kubota N, Kadowaki T, Murata M
Proceedings of the National Academy of Sciences of the United States of America 2015 Mar 10;112(10):E1067-76

Pyruvate kinase M2, but not M1, allele maintains immature metabolic states of murine embryonic stem cells.

Konno M, Ishii H, Koseki J, Tanuma N, Nishida N, Kawamoto K, Nishimura T, Nakata A, Matsui H, Noguchi K, Ozaki M, Noguchi Y, Shima H, Gotoh N, Nagano H, Doki Y, Mori M
Regenerative therapy 2015 Jun;1:63-71

Cancer associated fibroblasts transfer lipids and proteins to cancer cells through cargo vesicles supporting tumor growth.

Santi A, Caselli A, Ranaldi F, Paoli P, Mugnaioni C, Michelucci E, Cirri P
Biochimica et biophysica acta 2015 Dec;1853(12):3211-23

A small subunit processome protein promotes cancer by altering translation.

Yang HW, Kim TM, Song SS, Menon L, Jiang X, Huang W, Black PM, Park PJ, Carroll RS, Johnson MD
Oncogene 2015 Aug 20;34(34):4471-81

Metabolic effects of acute thiamine depletion are reversed by rapamycin in breast and leukemia cells.

Liu S, Miriyala S, Keaton MA, Jordan CT, Wiedl C, Clair DK, Moscow JA
PloS one 2014;9(1):e85702

Estradiol-17β upregulates pyruvate kinase M2 expression to coactivate estrogen receptor-α and to integrate metabolic reprogramming with the mitogenic response in endometrial cells.

Salama SA, Mohammad MA, Diaz-Arrastia CR, Kamel MW, Kilic GS, Ndofor BT, Abdel-Baki MS, Theiler SK
The Journal of clinical endocrinology and metabolism 2014 Oct;99(10):3790-9

Embelin inhibits endothelial mitochondrial respiration and impairs neoangiogenesis during tumor growth and wound healing.

Coutelle O, Hornig-Do HT, Witt A, Andree M, Schiffmann LM, Piekarek M, Brinkmann K, Seeger JM, Liwschitz M, Miwa S, Hallek M, Krönke M, Trifunovic A, Eming SA, Wiesner RJ, Hacker UT, Kashkar H
EMBO molecular medicine 2014 May;6(5):624-39

The lack of evidence for correlation of pyruvate kinase M2 expression with tumor grade in non-small cell lung cancer.

Kobierzycki C, Pula B, Werynska B, Piotrowska A, Muszczynska-Bernhard B, Dziegiel P, Rakus D
Anticancer research 2014 Jul;34(7):3811-7

Pyruvate kinase M2 expression, but not pyruvate kinase activity, is up-regulated in a grade-specific manner in human glioma.

Mukherjee J, Phillips JJ, Zheng S, Wiencke J, Ronen SM, Pieper RO
PloS one 2013;8(2):e57610

Reverse Warburg effect in a patient with aggressive B-cell lymphoma: is lactic acidosis a paraneoplastic syndrome?

Martinez-Outschoorn UE, Whitaker-Menezes D, Valsecchi M, Martinez-Cantarin MP, Dulau-Florea A, Gong J, Howell A, Flomenberg N, Pestell RG, Wagner J, Arana-Yi C, Sharma M, Sotgia F, Lisanti MP
Seminars in oncology 2013 Aug;40(4):403-18

Manipulation of PK-M mutually exclusive alternative splicing by antisense oligonucleotides.

Wang Z, Jeon HY, Rigo F, Bennett CF, Krainer AR
Open biology 2012 Oct;2(10):120133

Pyruvate kinase expression (PKM1 and PKM2) in cancer-associated fibroblasts drives stromal nutrient production and tumor growth.

Chiavarina B, Whitaker-Menezes D, Martinez-Outschoorn UE, Witkiewicz AK, Birbe R, Howell A, Pestell RG, Smith J, Daniel R, Sotgia F, Lisanti MP
Cancer biology & therapy 2011 Dec 15;12(12):1101-13

The reverse Warburg effect: glycolysis inhibitors prevent the tumor promoting effects of caveolin-1 deficient cancer associated fibroblasts.

Bonuccelli G, Whitaker-Menezes D, Castello-Cros R, Pavlides S, Pestell RG, Fatatis A, Witkiewicz AK, Vander Heiden MG, Migneco G, Chiavarina B, Frank PG, Capozza F, Flomenberg N, Martinez-Outschoorn UE, Sotgia F, Lisanti MP
Cell cycle (Georgetown, Tex.) 2010 May 15;9(10):1960-71

Oxidative stress in cancer associated fibroblasts drives tumor-stroma co-evolution: A new paradigm for understanding tumor metabolism, the field effect and genomic instability in cancer cells.

Martinez-Outschoorn UE, Balliet RM, Rivadeneira DB, Chiavarina B, Pavlides S, Wang C, Whitaker-Menezes D, Daumer KM, Lin Z, Witkiewicz AK, Flomenberg N, Howell A, Pestell RG, Knudsen ES, Sotgia F, Lisanti MP
Cell cycle (Georgetown, Tex.) 2010 Aug 15;9(16):3256-76

The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth.

Christofk HR, Vander Heiden MG, Harris MH, Ramanathan A, Gerszten RE, Wei R, Fleming MD, Schreiber SL, Cantley LC
Nature 2008 Mar 13;452(7184):230-3

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15821-1-AP(PK-M1-specific antibody) at dilution of 1:1500
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The PKM1-specific antibody from Proteintech is a rabbit polyclonal antibody to a peptide of human PKM1-specific. This antibody recognizes human, mouse, rat antigen. The PKM1-specific antibody has been validated for the following applications: ELISA, WB, IHC, IP, IF analysis.
Appendix: 15821-1-AP.pdf

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-PK-M1-specific (IP:15821-1-AP, 3ug; Detection:15821-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human skeletal muscle using 15821-1-AP(PK-M1-specific antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human skeletal muscle using 15821-1-AP(PK-M1-specific antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue