Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- HPA029501 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA029501, RRID:AB_10602118
- Product name
- Anti-PKM
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human PKM, Gene description: pyruvate kinase, muscle, Alternative Gene Names: OIP3, PK3, PKM2, THBP1, Validated applications: ICC, IHC, WB, Uniprot ID: P14618, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Proteomic Characterization of Colorectal Cancer Tissue from Patients Identifies Novel Putative Protein Biomarkers.
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Toponostics of invasive ductal breast carcinoma: combination of spatial protein expression imaging and quantitative proteome signature analysis.
Ludvigsen M, Thorlacius-Ussing L, Vorum H, Stender MT, Thorlacius-Ussing O, Honoré B
Current issues in molecular biology 2021 Sep 2;43(2):1043-1056
Current issues in molecular biology 2021 Sep 2;43(2):1043-1056
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C, Rexhepaj E, Singan V, Murphy R, Pepperkok R, Uhlén M, Simpson J, Lundberg E
Nature Methods 2013;10(4):315-323
Nature Methods 2013;10(4):315-323
Toponostics of invasive ductal breast carcinoma: combination of spatial protein expression imaging and quantitative proteome signature analysis.
Röwer C, Ziems B, Radtke A, Schmitt O, Reimer T, Koy C, Thiesen HJ, Gerber B, Glocker MO
International journal of clinical and experimental pathology 2011 Mar 31;4(5):454-67
International journal of clinical and experimental pathology 2011 Mar 31;4(5):454-67
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PKM antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Sample type
- Human
- Protocol
- Protocol