Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- bs-3334R - Provider product page
- Provider
- Bioss
- Proper citation
- Bioss Cat#bs-3334R, RRID:AB_10855824
- Product name
- PKM2 (Tyr105) Polyclonal Antibody
- Antibody type
- Polyclonal
- Description
- KLH conjugated synthetic phosphopeptide derived from human PKM2 around the phosphorylation site of Tyr105
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1ug/ul
- Storage
- Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
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Supportive validation
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- Protein: mouse liver lysates, 30ug; Primary: Anti-Phospho-PKM2(Tyr105) (bs-3334R) at 1:300; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; ECL excitated the fluorescence; Predicted band size : 58kD Observed band size : 58kD
- Sample type
- Mouse
- Other comments
- Liver
Supportive validation
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin embedded human liver carcinoma labeled with Anti-Phospho-PKM2 (Tyr105)Polyclonal Antibody, Unconjugated (bs-3334R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
- Sample type
- Human
- Other comments
- Liver