GTX107681

antibody from GeneTex

Targeting: PKM OIP3, PK3, PKM2, THBP1

Antibody data

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Validation data

PKM protein structure - GTX107681 shown in red.

Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107681) diluted at 1:500.
KD Reagent Type: siRNA
Downregulation: >50%
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
Antibody Lot Number: 40009

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX107681 diluted at 1:1000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Pyruvate Kinase (muscle) antibody detects PKM2 protein by Western blot analysis.A. 50 ?g Rat brain lysate/extract7.5 % SDS-PAGEPyruvate Kinase (muscle) antibody (GTX107681) dilution: 1:1000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample(30£gg whole cell lysate)A: 293TB: A431 (GTX27909)C: H1299D: HeLa S3 (GTX14654)E: Hep G2 (GTX27900)F: MOLT4 (GTX27912)G: Raji (GTX27908)7.5% SDS PAGEGTX107681 diluted at 1:1000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107681) diluted at 1:500.