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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [13]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX107977 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX107977, RRID:AB_1951264
- Product name
- PKM antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Proteomic investigating the cooperative lethal effect of EGFR and MDM2 inhibitors on ovarian carcinoma.
Localized hypoxia links ER stress to lung fibrosis through induction of C/EBP homologous protein.
Behavioral extremes of trait anxiety in mice are characterized by distinct metabolic profiles
Identification of up- and down-regulated proteins in doxorubicin-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of doxorubicin-associated resistance.
Chang SJ, Liao EC, Yeo HY, Kuo WH, Chen HY, Tsai YT, Wei YS, Chen YJ, Wang YS, Li JM, Shih CC, Chan CH, Lai ZY, Chou HC, Chuang YJ, Chan HL
Archives of biochemistry and biophysics 2018 Jun 1;647:10-32
Archives of biochemistry and biophysics 2018 Jun 1;647:10-32
Localized hypoxia links ER stress to lung fibrosis through induction of C/EBP homologous protein.
Burman A, Kropski JA, Calvi CL, Serezani AP, Pascoalino BD, Han W, Sherrill T, Gleaves L, Lawson WE, Young LR, Blackwell TS, Tanjore H
JCI insight 2018 Aug 23;3(16)
JCI insight 2018 Aug 23;3(16)
Behavioral extremes of trait anxiety in mice are characterized by distinct metabolic profiles
Filiou M, Asara J, Nussbaumer M, Teplytska L, Landgraf R, Turck C
Journal of Psychiatric Research 2014 November;58
Journal of Psychiatric Research 2014 November;58
Identification of up- and down-regulated proteins in doxorubicin-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of doxorubicin-associated resistance.
May EW, Lin ST, Lin CC, Chang JF, Hung E, Lo YW, Lin LH, Hu RY, Feng CL, Lin DY, Wu SB, Lee WC, Lyu KW, Chou HC, Chan HL
Pharmacological research 2014 Dec;90:1-17
Pharmacological research 2014 Dec;90:1-17
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107977) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE GTX107977 diluted at 1:10000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Pyruvate Kinase (muscle) antibody detects PKM2 protein by Western blot analysis.A. 50 µg rat brain lysate/extract7.5 % SDS-PAGEPyruvate Kinase (muscle) antibody (GTX107977) dilution: 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate)A: A431 (GTX27909)B: H1299C: Hela S37.5% SDS PAGEGTX107977 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Pyruvate Kinase (muscle) antibody detects Pyruvate Kinase (muscle) protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Pyruvate Kinase (muscle) antibody (GTX107977) diluted by 1:1000.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Pyruvate Kinase (muscle) antibody detects Pyruvate Kinase (muscle) protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Pyruvate Kinase (muscle) antibody (GTX107977) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Pyruvate Kinase antibody detects Pyruvate Kinase protein by western blot analysis. Mouse tissue extracts (50 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Pyruvate Kinase antibody (GTX107977) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Pyruvate Kinase antibody detects Pyruvate Kinase protein by western blot analysis. Rat tissue extracts (50 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Pyruvate Kinase antibody (GTX107977) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Pyruvate Kinase (muscle) antibody detects Pyruvate Kinase (muscle) protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and blotted with Pyruvate Kinase (muscle) antibody (GTX107977) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107977) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Pyruvate Kinase (muscle) antibody detects Pyruvate Kinase (muscle) protein by Western blot analysis. Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Pyruvate Kinase (muscle) antibody (GTX107977) diluted by 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Pyruvate Kinase (muscle) antibody detects Pyruvate Kinase (muscle) protein by Western blot analysis. Mouse tissue extracts (50 £gg) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with Pyruvate Kinase (muscle) antibody (GTX107977) diluted by 1:2000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Pyruvate Kinase (muscle) antibody detects Pyruvate Kinase (muscle) protein by Western blot analysis. Rat tissue extracts (50 £gg) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with Pyruvate Kinase (muscle) antibody (GTX107977) diluted by 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Pyruvate Kinase (muscle) antibody detects Pyruvate Kinase (muscle) protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Pyruvate Kinase (muscle) protein stained by Pyruvate Kinase (muscle) antibody (GTX107977) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of PKM2 protein from 293T whole cell extracts using 5 £gg of Pyruvate Kinase (muscle) antibody (GTX107977).Western blot analysis was performed using Pyruvate Kinase (muscle) antibody (GTX107977).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Pyruvate Kinase (muscle) antibody detects Pyruvate Kinase (muscle) protein at cytosol on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. Pyruvate Kinase (muscle) antibody (GTX107977) dilution: 1:500.
