GTX111319

antibody from GeneTex

Targeting: PKM OIP3, PK3, PKM2, THBP1

Antibody data

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Validation data

PKM protein structure - GTX111319 shown in red.

Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX111319) diluted at 1:500.
KD Reagent Type: siRNA
Downregulation: >75%
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
KD Reagent Prod page: KD Reagent Prod page
Antibody Lot Number: 40100

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX111319 diluted at 1:1000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Pyruvate Kinase (muscle) antibody detects PKM2 protein by Western blot analysis.A. 50 ?g Rat muscle lysate/extract7.5 % SDS-PAGEPyruvate Kinase (muscle) antibody (GTX111319) dilution: 1:1000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ug of whole cell lysate) A: A431 (GTX27909) B: H1299 7.5% SDS PAGE GTX111319 diluted at 1:1000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX111319) diluted at 1:500.