Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX111604 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111604, RRID:AB_11176552
- Product name
- PKM antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX111604) diluted at 1:10000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE GTX111604 diluted at 1:5000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Pyruvate Kinase (muscle) antibody detects PKM2 protein by Western blot analysis.A. 50 µg Rat muscle lysate/extract7.5 % SDS-PAGEPyruvate Kinase (muscle) antibody (GTX111604) dilution: 1:10000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX111604) diluted at 1:5000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX111604) diluted at 1:10000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed A431, using Pyruvate Kinase (muscle)(GTX111604) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PKM antibody detects PKM protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PKM protein stained by PKM antibody (GTX111604) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Pyruvate Kinase (muscle) antibody detects PKM2 protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. Pyruvate Kinase (muscle) antibody (GTX111604) dilution: 1:500.