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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PA5-34779 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PKM2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, H1299, mouse brain, rat muscle, HeLa S3, 293T. Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (88%), Rabbit (97%), Chicken (87%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.48 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PKM2 Polyclonal Antibody (Product # PA5-34779). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: H1299 . 7.5% SDS PAGE. PKM2 Polyclonal Antibody (Product # PA5-34779) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Pyruvate Kinase (muscle) antibody detects PKM2 protein by Western blot analysis. A. 50 µg Rat muscle lysate/extract.7.5 % SDS-PAGE. Pyruvate Kinase (muscle) antibody PKM2 Polyclonal Antibody (Product # PA5-34779) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PKM2 Polyclonal Antibody (Product # PA5-34779). Sample (50 µg of whole cell lysate). Lane A: Mouse brain . 7.5% SDS PAGE. PKM2 Polyclonal Antibody (Product # PA5-34779) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Pyruvate Kinase (muscle) (Product # PA5-34779) antibody at 1:100 dilution.
