Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-37684 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-PKM2 (Ser37) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for ICC/IF is MEF cells.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Podocyte specific deletion of PKM2 ameliorates LPS-induced podocyte injury through beta-catenin.
Cdc25A inhibits autophagy-mediated ferroptosis by upregulating ErbB2 through PKM2 dephosphorylation in cervical cancer cells.
PKM2-Induced the Phosphorylation of Histone H3 Contributes to EGF-Mediated PD-L1 Transcription in HCC.
Alquraishi M, Chahed S, Alani D, Puckett DL, Dowker PD, Hubbard K, Zhao Y, Kim JY, Nodit L, Fatima H, Donohoe D, Voy B, Chowanadisai W, Bettaieb A
Cell communication and signaling : CCS 2022 May 30;20(1):76
Cell communication and signaling : CCS 2022 May 30;20(1):76
Cdc25A inhibits autophagy-mediated ferroptosis by upregulating ErbB2 through PKM2 dephosphorylation in cervical cancer cells.
Wang C, Zeng J, Li LJ, Xue M, He SL
Cell death & disease 2021 Nov 6;12(11):1055
Cell death & disease 2021 Nov 6;12(11):1055
PKM2-Induced the Phosphorylation of Histone H3 Contributes to EGF-Mediated PD-L1 Transcription in HCC.
Wang X, Liang C, Yao X, Yang RH, Zhang ZS, Liu FY, Li WQ, Pei SH, Ma J, Xie SQ, Fang D
Frontiers in pharmacology 2020;11:577108
Frontiers in pharmacology 2020;11:577108
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot in-vitro kinase assay using purifed active ERK2, wild-type (WT) PKM2 and PKM2 S37A mutant. Detection was performed using PKM2 (pSer37) polyclonal antibody (Product # PA5-37684).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Phospho-PKM2 (Ser37) in methanol-fixed MEF cells, using Phospho-PKM2 (Ser37) Polyclonal Antibody (Product # PA5-37684).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 Cdc25A dephosphorylated PKM2 to suppress sorafenib-induced ferroptosis. A Relative p-PKM2 Ser37 levels in the nucleus and cytosol of transfected cells following sorafenib treatment. B Co-IP was used to analyse the interaction of Cdc25A and PKM2 in the nucleus and cytosol. C Relative p-PKM2 Ser37 levels in transfected cells with or without sorafenib treatment. D The MTT assay to analyse the viability of transfected cells following sorafenib treatment. E Cellular MDA levels in transfected cells after sorafenib treatment. F Cellular ROS levels in transfected cells after sorafenib treatment. G Cellular GSH levels in transfected cells after sorafenib treatment. H Cellular iron levels in transfected cells after sorafenib treatment. Each experiment was repeated three times. * P < 0.05; ** P < 0.01; *** P < 0.001.