Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA324317 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal Pyruvate Kinase (PKM2) Antibody (C-term)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal Pyruvate Kinase (PKM2) Antibody (C-term)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- PKM
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 2.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of anti-h PKM2-N491 Pab (Cat. #TA324317) in Ramos cell line lysates (35ug/lane). hPKM2-N491(arrow) was detected using the purified Pab.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of MCF7 cells stained with Pyruvate Kinase (PKM2) (C-term) antibody. MCF7 cells were incubated with TA324317 Pyruvate Kinase (PKM2) (C-term) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . Note the highly specific localization of the Pyruvate Kinase (PKM2) mainly to the cytoplasm.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Pyruvate Kinase (PKM2) Antibody (C-term) (Cat. #TA324317) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Pyruvate Kinase (PKM2) Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC