Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-C408221 - Provider product page
- Provider
- LSBio
- Product name
- PKM / Pyruvate Kinase, Muscle Antibody LS-C408221
- Antibody type
- Monoclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using PKM antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
