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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- F40140 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PKM2 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 476-505 from the human protein was used as the immunogen for this PKM2 antibody.
- Description
- Purified antibody
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the PKM2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent confocal image of MCF7 cells stained with PKM2 antibody. Alexa Fluor 488 conjugated donkey anti-rabbit secondary Ab (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). PKM2 is localized to the cytoplasm.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- PKM2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma.
