Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AM50349PU-N - Provider product page
- Provider
- OriGene
- Product name
- PKM2 (PKM) mouse monoclonal antibody, clone AT1B10, Purified
- Antibody type
- Monoclonal
- Description
- PKM2 (PKM) mouse monoclonal antibody, clone AT1B10, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PKM
- Isotype
- IgG
- Antibody clone number
- AT1B10
- Vial size
- 100 µl
- Concentration
- 1.0 mg/ml
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- Fig.1: The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HeLa cell lysate Lane 2 : Jurkat cell lysate Lane 3 : MCF7 cell lysate Lane 4 : A549 cell lysate Lane 5 : 293T cell lysate Fig 2: The Recombinant human PKM2 (50ng) protein was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Validation comment
- WB