AM50349PU-S

antibody from OriGene

Targeting: PKM OIP3, PK3, PKM2, THBP1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Antibody Data

Product number

AM50349PU-S

Provider

OriGene

Product name

PKM2 (PKM) mouse monoclonal antibody, clone AT1B10, Purified

Antibody type

Monoclonal

Description

PKM2 (PKM) mouse monoclonal antibody, clone AT1B10, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

PKM

Isotype

IgG

Antibody clone number

AT1B10

Vial size

50 µl

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Fig.1: The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HeLa cell lysate Lane 2 : Jurkat cell lysate Lane 3 : MCF7 cell lysate Lane 4 : A549 cell lysate Lane 5 : 293T cell lysate Fig 2: The Recombinant human PKM2 (50ng) protein was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Validation comment

WB