Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB4574 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4574, RRID:AB_1578848
- Product name
- PKM2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PKM2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Using the yeast two-hybrid system to identify human epithelial cell proteins that bind gonococcal Opa proteins: intracellular gonococci bind pyruvate kinase via their Opa proteins and require host pyruvate for growth.
A pancreatic cancer-specific expression profile.
Cytosolic thyroid hormone-binding protein is a monomer of pyruvate kinase.
Williams JM, Chen GC, Zhu L, Rest RF
Molecular microbiology 1998 Jan;27(1):171-86
Molecular microbiology 1998 Jan;27(1):171-86
A pancreatic cancer-specific expression profile.
Gress TM, Müller-Pillasch F, Geng M, Zimmerhackl F, Zehetner G, Friess H, Büchler M, Adler G, Lehrach H
Oncogene 1996 Oct 17;13(8):1819-30
Oncogene 1996 Oct 17;13(8):1819-30
Cytosolic thyroid hormone-binding protein is a monomer of pyruvate kinase.
Kato H, Fukuda T, Parkison C, McPhie P, Cheng SY
Proceedings of the National Academy of Sciences of the United States of America 1989 Oct;86(20):7861-5
Proceedings of the National Academy of Sciences of the United States of America 1989 Oct;86(20):7861-5
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The PKM2 polyclonal antibody (Cat # PAB4574) is used in Western blot to detect PKM2 in HeLa cell lysate .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- A : Negative control of HeLa cells without Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L).Alexa-Fluor-546 emits orange fluorescence.B : Immunofluorescence analysis of PKM2 polyclonal antibody (Cat # PAB4574) in HeLa cells. 0.025 mg/mL primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L).
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with PKM2 polyclonal antibody (Cat # PAB4574) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)