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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MAB9714 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB9714, RRID:AB_10754079
- Product name
- PKM2 monoclonal antibody, clone AT4M3
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein corresponding to amino acids 1-531 of human PKM2.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 μl
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of cell lysates (35 ug) with PKM2 monoclonal antibody, clone AT4M3 (Cat # MAB9714) at 1 : 500 dilution. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : NIH/3T3.Lane 2 : Jurkat.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and PKM2 monoclonal antibody, clone AT4M3 (Cat # MAB9714) at 1 : 500 dilution with Texas Red (Red).
- Validation comment
- Immunofluorescence
