Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- H00005315-D01P - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005315-D01P, RRID:AB_1503932
- Product name
- Rabbit Polyclonal PKM2 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A purified. PKM2 - pyruvate kinase, muscle,
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1.
Two-tiered approach identifies a network of cancer and liver disease-related genes regulated by miR-122.
Luo W, Hu H, Chang R, Zhong J, Knabel M, O'Meally R, Cole RN, Pandey A, Semenza GL
Cell 2011 May 27;145(5):732-44
Cell 2011 May 27;145(5):732-44
Two-tiered approach identifies a network of cancer and liver disease-related genes regulated by miR-122.
Boutz DR, Collins PJ, Suresh U, Lu M, RamÃrez CM, Fernández-Hernando C, Huang Y, Abreu Rde S, Le SY, Shapiro BA, Liu AM, Luk JM, Aldred SF, Trinklein ND, Marcotte EM, Penalva LO
The Journal of biological chemistry 2011 May 20;286(20):18066-78
The Journal of biological chemistry 2011 May 20;286(20):18066-78
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PKM2 Antibody [H00005315-D01P] - Analysis of PKM2 expression in transfected 293T cell line by PKM2 polyclonal antibody.Lane 1: PKM2 transfected lysate(57.90 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PKM2 Antibody [H00005315-D01P] - Western Blot analysis of HeLa lysates using H00005315-D01P. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).