Antibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP2-14833 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal PKM1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is specific for the M1 isoform of PKM.
- Reactivity
- Human, Mouse, Bovine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.17 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The effect of HIF-1α and PKM1 expression on acquisition of chemoresistance.
NOX4 functions as a mitochondrial energetic sensor coupling cancer metabolic reprogramming to drug resistance.
MiR-145 negatively regulates Warburg effect by silencing KLF4 and PTBP1 in bladder cancer cells.
A Novel Combination RNAi toward Warburg Effect by Replacement with miR-145 and Silencing of PTBP1 Induces Apoptotic Cell Death in Bladder Cancer Cells.
M-Type Pyruvate Kinase Isoforms and Lactate Dehydrogenase A in the Mammalian Retina: Metabolic Implications.
MiR-133b inhibits growth of human gastric cancer cells by silencing pyruvate kinase muscle-splicer polypyrimidine tract-binding protein 1.
Positive feedback of DDX6/c-Myc/PTB1 regulated by miR-124 contributes to maintenance of the Warburg effect in colon cancer cells.
MicroRNA-124 inhibits cancer cell growth through PTB1/PKM1/PKM2 feedback cascade in colorectal cancer.
Pyruvate kinase M2 regulates Hif-1α activity and IL-1β induction and is a critical determinant of the warburg effect in LPS-activated macrophages.
Mitochondrial integrity in a neonatal bovine model of right ventricular dysfunction.
Breast-cancer-secreted miR-122 reprograms glucose metabolism in premetastatic niche to promote metastasis.
Organ-specific PTB1-associated microRNAs determine expression of pyruvate kinase isoforms.
Anti-cancer fatty-acid derivative induces autophagic cell death through modulation of PKM isoform expression profile mediated by bcr-abl in chronic myeloid leukemia.
Okazaki M, Fushida S, Tsukada T, Kinoshita J, Oyama K, Miyashita T, Ninomiya I, Harada S, Ohta T
Cancer management and research 2018;10:1865-1874
Cancer management and research 2018;10:1865-1874
NOX4 functions as a mitochondrial energetic sensor coupling cancer metabolic reprogramming to drug resistance.
Shanmugasundaram K, Nayak BK, Friedrichs WE, Kaushik D, Rodriguez R, Block K
Nature communications 2017 Oct 19;8(1):997
Nature communications 2017 Oct 19;8(1):997
MiR-145 negatively regulates Warburg effect by silencing KLF4 and PTBP1 in bladder cancer cells.
Minami K, Taniguchi K, Sugito N, Kuranaga Y, Inamoto T, Takahara K, Takai T, Yoshikawa Y, Kiyama S, Akao Y, Azuma H
Oncotarget 2017 May 16;8(20):33064-33077
Oncotarget 2017 May 16;8(20):33064-33077
A Novel Combination RNAi toward Warburg Effect by Replacement with miR-145 and Silencing of PTBP1 Induces Apoptotic Cell Death in Bladder Cancer Cells.
Takai T, Yoshikawa Y, Inamoto T, Minami K, Taniguchi K, Sugito N, Kuranaga Y, Shinohara H, Kumazaki M, Tsujino T, Takahara K, Ito Y, Akao Y, Azuma H
International journal of molecular sciences 2017 Jan 17;18(1)
International journal of molecular sciences 2017 Jan 17;18(1)
M-Type Pyruvate Kinase Isoforms and Lactate Dehydrogenase A in the Mammalian Retina: Metabolic Implications.
Casson RJ, Wood JP, Han G, Kittipassorn T, Peet DJ, Chidlow G
Investigative ophthalmology & visual science 2016 Jan 1;57(1):66-80
Investigative ophthalmology & visual science 2016 Jan 1;57(1):66-80
MiR-133b inhibits growth of human gastric cancer cells by silencing pyruvate kinase muscle-splicer polypyrimidine tract-binding protein 1.
Sugiyama T, Taniguchi K, Matsuhashi N, Tajirika T, Futamura M, Takai T, Akao Y, Yoshida K
Cancer science 2016 Dec;107(12):1767-1775
Cancer science 2016 Dec;107(12):1767-1775
Positive feedback of DDX6/c-Myc/PTB1 regulated by miR-124 contributes to maintenance of the Warburg effect in colon cancer cells.
Taniguchi K, Sugito N, Kumazaki M, Shinohara H, Yamada N, Matsuhashi N, Futamura M, Ito Y, Otsuki Y, Yoshida K, Uchiyama K, Akao Y
Biochimica et biophysica acta 2015 Sep;1852(9):1971-80
Biochimica et biophysica acta 2015 Sep;1852(9):1971-80
MicroRNA-124 inhibits cancer cell growth through PTB1/PKM1/PKM2 feedback cascade in colorectal cancer.
Taniguchi K, Sugito N, Kumazaki M, Shinohara H, Yamada N, Nakagawa Y, Ito Y, Otsuki Y, Uno B, Uchiyama K, Akao Y
Cancer letters 2015 Jul 10;363(1):17-27
Cancer letters 2015 Jul 10;363(1):17-27
Pyruvate kinase M2 regulates Hif-1α activity and IL-1β induction and is a critical determinant of the warburg effect in LPS-activated macrophages.
Palsson-McDermott EM, Curtis AM, Goel G, Lauterbach MA, Sheedy FJ, Gleeson LE, van den Bosch MW, Quinn SR, Domingo-Fernandez R, Johnston DG, Jiang JK, Israelsen WJ, Keane J, Thomas C, Clish C, Vander Heiden M, Xavier RJ, O'Neill LA
Cell metabolism 2015 Jan 6;21(1):65-80
Cell metabolism 2015 Jan 6;21(1):65-80
Mitochondrial integrity in a neonatal bovine model of right ventricular dysfunction.
Bruns DR, Brown RD, Stenmark KR, Buttrick PM, Walker LA
American journal of physiology. Lung cellular and molecular physiology 2015 Jan 15;308(2):L158-67
American journal of physiology. Lung cellular and molecular physiology 2015 Jan 15;308(2):L158-67
Breast-cancer-secreted miR-122 reprograms glucose metabolism in premetastatic niche to promote metastasis.
Fong MY, Zhou W, Liu L, Alontaga AY, Chandra M, Ashby J, Chow A, O'Connor ST, Li S, Chin AR, Somlo G, Palomares M, Li Z, Tremblay JR, Tsuyada A, Sun G, Reid MA, Wu X, Swiderski P, Ren X, Shi Y, Kong M, Zhong W, Chen Y, Wang SE
Nature cell biology 2015 Feb;17(2):183-94
Nature cell biology 2015 Feb;17(2):183-94
Organ-specific PTB1-associated microRNAs determine expression of pyruvate kinase isoforms.
Taniguchi K, Ito Y, Sugito N, Kumazaki M, Shinohara H, Yamada N, Nakagawa Y, Sugiyama T, Futamura M, Otsuki Y, Yoshida K, Uchiyama K, Akao Y
Scientific reports 2015 Feb 27;5:8647
Scientific reports 2015 Feb 27;5:8647
Anti-cancer fatty-acid derivative induces autophagic cell death through modulation of PKM isoform expression profile mediated by bcr-abl in chronic myeloid leukemia.
Shinohara H, Taniguchi K, Kumazaki M, Yamada N, Ito Y, Otsuki Y, Uno B, Hayakawa F, Minami Y, Naoe T, Akao Y
Cancer letters 2015 Apr 28;360(1):28-38
Cancer letters 2015 Apr 28;360(1):28-38
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PKM1 Antibody [NBP2-14833] - PKM1 antibody was tested in 1. human skeletal muscle 2. mouse skeletal muscle 3. human brain 4. mouse brain and 5. human heart cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PKM1 Antibody [NBP2-14833] - Detection of PKM1 on HEK293T and U-87 cells. Lane 1: HEK293T cells (major isoform is PKM2, human). Lane 2: HEK293T cells with plasmid overexpressing mouse PKM1. Lane 3: HEK293T cells with plasmid overexpressing mouse PKM2. Lane 4: U-87 cells with control shRNA (major isoform is PKM2, human). Lane 5: U-87 cells with PTB/A1/A2 shRNAs (major isoform is PKM1, human). Photo courtesy of product review by verified customer.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: PKM1 Antibody [NBP2-14833] - Simple Western lane view shows a specific band for PKM1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PKM1 Antibody [NBP2-14833] - PKM1 antibody was tested in human breast cancer using DAB with hematoxylin counterstain.