- NBP1-48308 - Novus Biologicals PKM antibody

Submitted references Enhanced aerobic glycolysis of nasopharyngeal carcinoma cells by Epstein-Barr virus latent membrane protein 1.

Sung WW, Chen PR, Liao MH, Lee JW
Experimental cell research 2017 Oct 1;359(1):94-100

MiR-145 negatively regulates Warburg effect by silencing KLF4 and PTBP1 in bladder cancer cells.

Minami K, Taniguchi K, Sugito N, Kuranaga Y, Inamoto T, Takahara K, Takai T, Yoshikawa Y, Kiyama S, Akao Y, Azuma H
Oncotarget 2017 May 16;8(20):33064-33077

A Novel Combination RNAi toward Warburg Effect by Replacement with miR-145 and Silencing of PTBP1 Induces Apoptotic Cell Death in Bladder Cancer Cells.

Takai T, Yoshikawa Y, Inamoto T, Minami K, Taniguchi K, Sugito N, Kuranaga Y, Shinohara H, Kumazaki M, Tsujino T, Takahara K, Ito Y, Akao Y, Azuma H
International journal of molecular sciences 2017 Jan 17;18(1)

MiR-133b inhibits growth of human gastric cancer cells by silencing pyruvate kinase muscle-splicer polypyrimidine tract-binding protein 1.

Sugiyama T, Taniguchi K, Matsuhashi N, Tajirika T, Futamura M, Takai T, Akao Y, Yoshida K
Cancer science 2016 Dec;107(12):1767-1775

Positive feedback of DDX6/c-Myc/PTB1 regulated by miR-124 contributes to maintenance of the Warburg effect in colon cancer cells.

Taniguchi K, Sugito N, Kumazaki M, Shinohara H, Yamada N, Matsuhashi N, Futamura M, Ito Y, Otsuki Y, Yoshida K, Uchiyama K, Akao Y
Biochimica et biophysica acta 2015 Sep;1852(9):1971-80

Mitochondrial integrity in a neonatal bovine model of right ventricular dysfunction.

Bruns DR, Brown RD, Stenmark KR, Buttrick PM, Walker LA
American journal of physiology. Lung cellular and molecular physiology 2015 Jan 15;308(2):L158-67

Organ-specific PTB1-associated microRNAs determine expression of pyruvate kinase isoforms.

Taniguchi K, Ito Y, Sugito N, Kumazaki M, Shinohara H, Yamada N, Nakagawa Y, Sugiyama T, Futamura M, Otsuki Y, Yoshida K, Uchiyama K, Akao Y
Scientific reports 2015 Feb 27;5:8647

GLUT3 and PKM2 regulate OCT4 expression and support the hypoxic culture of human embryonic stem cells.

Christensen DR, Calder PC, Houghton FD
Scientific reports 2015 Dec 7;5:17500

Anti-cancer fatty-acid derivative induces autophagic cell death through modulation of PKM isoform expression profile mediated by bcr-abl in chronic myeloid leukemia.

Shinohara H, Taniguchi K, Kumazaki M, Yamada N, Ito Y, Otsuki Y, Uno B, Hayakawa F, Minami Y, Naoe T, Akao Y
Cancer letters 2015 Apr 28;360(1):28-38

Pyruvate kinase and aspartate-glutamate carrier distributions reveal key metabolic links between neurons and glia in retina.

Lindsay KJ, Du J, Sloat SR, Contreras L, Linton JD, Turner SJ, Sadilek M, Satrústegui J, Hurley JB
Proceedings of the National Academy of Sciences of the United States of America 2014 Oct 28;111(43):15579-84

Divergent LIN28-mRNA associations result in translational suppression upon the initiation of differentiation.

Tan SM, Altschuler G, Zhao TY, Ang HS, Yang H, Lim B, Vardy L, Hide W, Thomson AM, Lareu RR
Nucleic acids research 2014 Jul;42(12):7997-8007

Reduction of reactive oxygen species ameliorates metabolism-secretion coupling in islets of diabetic GK rats by suppressing lactate overproduction.

Sasaki M, Fujimoto S, Sato Y, Nishi Y, Mukai E, Yamano G, Sato H, Tahara Y, Ogura K, Nagashima K, Inagaki N
Diabetes 2013 Jun;62(6):1996-2003

Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1.

Luo W, Hu H, Chang R, Zhong J, Knabel M, O'Meally R, Cole RN, Pandey A, Semenza GL
Cell 2011 May 27;145(5):732-44

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Simple Western: PKM2 Antibody [NBP1-48308] - Lane view shows a specific band for PKM2 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: PKM2 Antibody [NBP1-48308] - Analysis of HeLa lysates using NBP1-48308. Image courtesy of Gregg Semenza, (PMID: 21620138).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: PKM2 Antibody [NBP1-48308] - Analysis of PKM2 in MCF7 whole cell lysates.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: PKM2 Antibody [NBP1-48308] - Expression profile of PKM isoforms in tissues from mouse organs. PKM1 and PKM2 were detected by Western blotting in under the same experimental conditions at the same time. The full-length blots are presented in Supplementary Figure S2b. Results are presented as the mean +/- SD (** P < 0.01). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep08647) licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: PKM2 Antibody [NBP1-48308] - Expression profile of PKM isoforms in various cancer cell lines, control cell lines, and cells in primary culture. PKM1 and PKM2 were detected by Western blotting under the same experimental conditions at the same time. The full-length blots are presented in Supplementary Figure S2a. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep08647) licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: PKM2 Antibody [NBP1-48308] - Expression of PKM1 and PKM2 in clinical colorectal cancer samples. The protein expression of PKM1 and PKM2 in clinical specimens of cancer tumor (T) and the adjacent normal tissues (N) is shown. PKM1 and PKM2 were detected by Western blotting in under the same experimental conditions at the same time. The full-length blots are presented in Supplementary Figure S3b. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep08647) licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: PKM2 Antibody [NBP1-48308] - Staining of PKM2 in mouse liver tissue.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: PKM2 Antibody [NBP1-48308] - An intracellular stain was performed on HeLa cells with PKM2 Antibody NBP1-48308AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: PKM2 Antibody [NBP1-48308] - An intracellular stain was performed on MCF7 cells with PKM2 Antibody NBP1-48308AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: PKM2 Antibody [NBP1-48308] - An intracellular stain was performed on Neuro2a cells with PKM2 Antibody NBP1-48308AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

NBP1-48308

Antibody data