- PA5-23034 - Invitrogen Antibodies PKM antibody

Submitted references Longitudinal in vivo bioimaging of hepatocyte transcription factor activity following cholestatic liver injury in mice.

Delhove JM, Buckley SM, Perocheau DP, Karda R, Arbuthnot P, Henderson NC, Waddington SN, McKay TR
Scientific reports 2017 Feb 3;7:41874

Breast cancer stem cells rely on fermentative glycolysis and are sensitive to 2-deoxyglucose treatment.

Ciavardelli D, Rossi C, Barcaroli D, Volpe S, Consalvo A, Zucchelli M, De Cola A, Scavo E, Carollo R, D'Agostino D, Forlì F, D'Aguanno S, Todaro M, Stassi G, Di Ilio C, De Laurenzi V, Urbani A
Cell death & disease 2014 Jul 17;5(7):e1336

p63 isoforms regulate metabolism of cancer stem cells.

D'Aguanno S, Barcaroli D, Rossi C, Zucchelli M, Ciavardelli D, Cortese C, De Cola A, Volpe S, D'Agostino D, Todaro M, Stassi G, Di Ilio C, Urbani A, De Laurenzi V
Journal of proteome research 2014 Apr 4;13(4):2120-36

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PKM2 using a polyclonal antibody (Product # PA5-23034).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PKM2 using a polyclonal antibody (Product # PA5-23034).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PKM2 using a polyclonal antibody (Product # PA5-23034).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PKM2 in MCF7 whole cell lysates. Sample was incubated in PKM2 polyclonal antibody (Product # PA5-23034).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PKM2 in 0.1 mg/mL HeLa lysate. Samples were incubated in PKM2 polyclonal (Product # PA5-23034). This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of PKM2 was achieved by transfecting MDA-MB-231 cells with PKM2 specific siRNAs (Silencer® select Product # s10574). Western blot analysis (Fig. a) was performed using whole cell extracts from the PKM2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with PKM2 Polyclonal Antibody (Product # PA5-23034, 0.5 µg/ml) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to PKM2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of DU 145 (Lane 1), MDA-MB-231 (Lane 2), Ramos (Lane 3) and NTERA-2 cl.D1 (Lane 4). The blot was probed with Anti-PKM2 Polyclonal Antibody (Product # PA5-23034, 0.5 µg/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 58 kDa band corresponding to PKM2 was observed across the cell lines tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of PKM2 in HeLa cells. Samples were incubated in PKM2 polyclonal antibody (Product # PA5-23034).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of PKM2 was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PKM2 Polyclonal Antibody (Product # PA5-23034) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of PKM2 in mouse liver tissue. Samples were incubated in PKM2 polyclonal antibody (Product # PA5-23034).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of PKM2 in HeLa cells (blue) and a matched isotype control (orange). Samples were incubated in PKM2 polyclonal antibody (Product # PA5-23034) using a dilution of 5 µg/mL for 30 minutes at room temperature. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Both antibodies were conjugated to Alexa Fluor 488.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Reporter gene expression is stable and restricted to hepatocytes after pBDL following neonatal intravascular administration of lentiviral vectors. SFFV-FLuc/eGFP lentivirus was administered to P1 neonatal mice by intravascular (i.v.) injection and then subjected to pBDL or sham pBDL in adulthood. ( A ) Mice were subjected to continued luciferase bioimaging over 40 days where no change in luciferase activity was observed over time or between pBDL and sham groups (n = 10 pBDL, 5 sham, not significant, Student's t-test). Mice were sacrificed 90 days after pBDL and co-immunostained for GFP and markers of ( Bi-iii ) hepatocytes; HNF4alpha, ( C-iii ) biliary epithelia; CK7, ( Di-iii ) hepatic progenitors; PKM2, ( Ei-iii ) myofibroblasts; alphaSMA and ( Fi-iii ) hepatic stellate cells; GFAP (all groups n = 3-6).

PA5-23034

Antibody data