Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-29339 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PKM2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, mouse brain, rat brain. Predicted reactivity: Mouse (97%), Rat (96%), Xenopus laevis (86%), Rabbit (97%), Chicken (87%), Bovine (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.03 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of Pyruvate Kinase (muscle) using 50 µg Rat muscle lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Pyruvate Kinase (muscle) polyclonal antibody (Product # PA5-29339) at a dilution of 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PKM2 was performed by separating 50 µg of various tissue extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a PKM2 Polyclonal Antibody (Product # PA5-29339) at a dilution of 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PKM2 Polyclonal Antibody (Product # PA5-29339). Sample (30 µg of whole cell lysate). Lane A: A431 . 7.5% SDS PAGE. PKM2 Polyclonal Antibody (Product # PA5-29339) diluted at 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of PKM2 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: PKM2 Polyclonal Antibody (Product # PA5-29339) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PKM2 Polyclonal Antibody detects PKM protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. PKM stained by PKM2 Polyclonal Antibody (Product # PA5-29339) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Pyruvate Kinase (muscle) antibody detects PKM2 protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. Pyruvate Kinase (muscle) antibody (Product # PA5-29339) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.