60268-1-Ig

antibody from Proteintech Group

Targeting: PKM OIP3, PK3, PKM2, THBP1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

60268-1-Ig

Provider

Proteintech Group

Product name

PKM2-specific antibody

Antibody type

Monoclonal

Description

KD/KO validated PKM2-specific antibody (Cat. #60268-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

1C11C7

Vial size

20ul, 150ul

References

Submitted references

Development of a one-plasmid system to replace the endogenous protein with point mutation for post-translational modification studies.

Ishrat I, Cheng A, Yu F, Guo J, Zhang P, Zhang K, Yang Z
Molecular biology reports 2022 Jan;49(1):1-7

---

Study on the Antitumor Effect and Glycolysis of Andrographolide in Anaplastic Thyroid Carcinoma.

Yang K, Wu K, Feng J, Yutian L, Zhu X, Xu D
Evidence-based complementary and alternative medicine : eCAM 2021;2021:5526581

---

Qian Yang Yu Yin Granule Improves Renal Injury of Hypertension by Regulating Metabolic Reprogramming Mediated by HIF-1α/PKM2 Positive Feedback Loop.

Qian L, Ren S, Xu Z, Zheng Y, Wu L, Yang Y, Wang Y, Li J, Yan S, Fang Z
Frontiers in pharmacology 2021;12:667433

---

Rapamycin facilitates differentiation of regulatory T cells via enhancement of oxidative phosphorylation.

Chen X, Li S, Long D, Shan J, Li Y
Cellular immunology 2021 Jul;365:104378

---

Shikonin differentially regulates glucose metabolism via PKM2 and HIF1α to overcome apoptosis in a refractory HCC cell line.

Yang W, Liu J, Hou L, Chen Q, Liu Y
Life sciences 2021 Jan 15;265:118796

---

Pyruvate kinase M2 (PKM2) interacts with activating transcription factor 2 (ATF2) to bridge glycolysis and pyroptosis in microglia.

Li M, Lu H, Wang X, Duan C, Zhu X, Zhang Y, Ge X, Ji F, Wang X, Su J, Zhang D
Molecular immunology 2021 Dec;140:250-266

---

Endoplasmic reticulum stress promotes sorafenib resistance via miR-188-5p/hnRNPA2B1-mediated upregulation of PKM2 in hepatocellular carcinoma.

Zhou B, Lu D, Wang A, Cui J, Zhang L, Li J, Fan L, Wei W, Liu J, Sun G
Molecular therapy. Nucleic acids 2021 Dec 3;26:1051-1065

---

Dihydroartemisinin Inhibits the Proliferation of Leukemia Cells K562 by Suppressing PKM2 and GLUT1 Mediated Aerobic Glycolysis.

Gao P, Shen S, Li X, Liu D, Meng Y, Liu Y, Zhu Y, Zhang J, Luo P, Gu L
Drug design, development and therapy 2020;14:2091-2100

---

Danqi Pill Protects Against Heart Failure Post-Acute Myocardial Infarction via HIF-1α/PGC-1α Mediated Glucose Metabolism Pathway.

Zhang Q, Guo D, Wang Y, Wang X, Wang Q, Wu Y, Li C, Wang W, Wang Y
Frontiers in pharmacology 2020;11:458

---

SQSTM1-dependent autophagic degradation of PKM2 inhibits the production of mature IL1B/IL-1β and contributes to LIPUS-mediated anti-inflammatory effect.

Zhang B, Chen H, Ouyang J, Xie Y, Chen L, Tan Q, Du X, Su N, Ni Z, Chen L
Autophagy 2020 Jul;16(7):1262-1278

---

Dietary fish oil supplement induces age-specific contractile and proteomic responses in muscles of male rats.

Russ DW, Dimova K, Morris E, Pacheco M, Garvey SM, Scordilis SP
Lipids in health and disease 2020 Jul 9;19(1):165

---

Simvastatin re-sensitizes hepatocellular carcinoma cells to sorafenib by inhibiting HIF-1α/PPAR-γ/PKM2-mediated glycolysis.

Feng J, Dai W, Mao Y, Wu L, Li J, Chen K, Yu Q, Kong R, Li S, Zhang J, Ji J, Wu J, Mo W, Xu X, Guo C
Journal of experimental & clinical cancer research : CR 2020 Jan 30;39(1):24

---

Supra-coronary aortic banding improves right ventricular function in experimental pulmonary arterial hypertension in rats by increasing systolic right coronary artery perfusion.

Tian L, Xiong PY, Alizadeh E, Lima PDA, Potus F, Mewburn J, Martin A, Chen KH, Archer SL
Acta physiologica (Oxford, England) 2020 Aug;229(4):e13483

---

PKM2 is the target of proanthocyanidin B2 during the inhibition of hepatocellular carcinoma.

Feng J, Wu L, Ji J, Chen K, Yu Q, Zhang J, Chen J, Mao Y, Wang F, Dai W, Xu L, Wu J, Guo C
Journal of experimental & clinical cancer research : CR 2019 May 17;38(1):204

---

miR-122 inhibits cancer cell malignancy by targeting PKM2 in gallbladder carcinoma.

Lu W, Zhang Y, Zhou L, Wang X, Mu J, Jiang L, Hu Y, Dong P, Liu Y
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2015 Nov 6;

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Western blot analysis of PK-M2-specific in various tissues and cell lines using Proteintech antibody 60268-1-Ig at dilution of 1:1000

Sample type

tissue

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The PK-M2-specific antibody from Proteintech is a mouse monoclonal antibody to a peptide of human PK-M2-specific. This antibody recognizes human,mouse, rat antigen. The PK-M2-specific antibody has been validated for the following applications: ELISA, IHC, IF, WB analysis.

Appendix

60268-1-Ig.pdf

Show more

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human colon cancer using 60268-1-Ig(PK-M2-specific antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human colon cancer using 60268-1-Ig(PK-M2-specific antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue