60268-1-Ig

antibody from Proteintech Group

Targeting: PKM OIP3, PK3, PKM2, THBP1

Provider product page for 60268-1-Ig

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [48]
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Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 60268-1-Ig - Provider product page
Provider: Proteintech Group
Product name: PKM2-specific antibody
Antibody type: Monoclonal
Description: KD/KO validated PKM2-specific antibody (Cat. #60268-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Mouse
Conjugate: Unconjugated
Isotype: IgG
Antibody clone number: 1C11C7
Vial size: 20ul, 150ul

PKM protein structure - 60268-1-Ig shown in red.

Submitted references Exploration of Nitrotyrosine-Containing Proteins and Peptides by Antibody-Based Enrichment Strategies.

Bhat FA, Mangalaparthi KK, Ding H, Jain A, Hsu JS, Peterson JA, Zenka RM, Mun DG, Kandasamy RK, Pandey A
Molecular & cellular proteomics : MCP 2024 Mar;23(3):100733

Augmentation of scleral glycolysis promotes myopia through histone lactylation.

Lin X, Lei Y, Pan M, Hu C, Xie B, Wu W, Su J, Li Y, Tan Y, Wei X, Xue Z, Xu R, Di M, Deng H, Liu S, Yang X, Qu J, Chen W, Zhou X, Zhao F
Cell metabolism 2024 Mar 5;36(3):511-525.e7

Targeting PKM2 improves the gemcitabine sensitivity of intrahepatic cholangiocarcinoma cells via inhibiting β-catenin signaling pathway.

Yu W, Zeng F, Xiao Y, Chen L, Qu H, Hong J, Qu C, Cheng G
Chemico-biological interactions 2024 Jan 5;387:110816

TIMELESS promotes reprogramming of glucose metabolism in oral squamous cell carcinoma.

Chen Y, Han Z, Zhang L, Gao C, Wei J, Yang X, Han Y, Li Y, Zhang C, Wei Y, Dong J, Xun W, Sun W, Zhang T, Zhang H, Chen J, Yuan P
Journal of translational medicine 2024 Jan 4;22(1):21

Candida albicans Infection Disrupts the Metabolism of Vaginal Epithelial Cells and Inhibits Cellular Glycolysis.

Zhao Y, Wang P, Sun X, Zhao M, Chen Y, Gao X
Microorganisms 2024 Jan 30;12(2)

Inhibition of sphingosine kinase 1 attenuates LPS-induced acute lung injury by suppressing endothelial cell pyroptosis.

Li S, Xue X, Zhang H, Jiang L, Zhang Y, Zhu X, Wang Y
Chemico-biological interactions 2024 Feb 25;390:110868

Protein 4.1R regulates M1 macrophages polarization via glycolysis, alleviating sepsis-induced liver injury in mice.

Sang SY, Wang YJ, Liang T, Liu Y, Liu JJ, Li H, Liu X, Kang QZ, Wang T
International immunopharmacology 2024 Feb 15;128:111546

TRIB3 promotes the growth of oral squamous cell carcinoma by regulating JNK/JUN-mediated aerobic glycolysis.

Meng Z, Wang Y, Wang X, Han X
Archives of oral biology 2024 Aug;164:105977

The relationship of ALPK1, hyaluronic acid and M1 macrophage polarization in the temporomandibular joint synovitis.

Zhao J, Feng Y, Liu X, Li H, Guo H, Ke J, Long X
Journal of cellular and molecular medicine 2024 Apr;28(7):e18172

Identification of PKM2 as a pyroptosis-related key gene aggravates senile osteoporosis via the NLRP3/Caspase-1/GSDMD signaling pathway.

Li Z, Wang B, Wang R, Zhang Z, Xiong J, Wang X, Ma Y, Han L
The international journal of biochemistry & cell biology 2024 Apr;169:106537

Leech extract alleviates idiopathic pulmonary fibrosis by TGF-β1/Smad3 signaling pathway.

Zhang Y, Lu YB, Zhu WJ, Gong XX, Qian R, Lu YJ, Li Y, Yao WF, Bao BH, Zhang Y, Zhang L, Cheng FF
Journal of ethnopharmacology 2024 Apr 24;324:117737

The diapause-like colorectal cancer cells induced by SMC4 attenuation are characterized by low proliferation and chemotherapy insensitivity.

Sun X, He L, Liu H, Thorne RF, Zeng T, Liu L, Zhang B, He M, Huang Y, Li M, Gao E, Ma M, Cheng C, Meng F, Lang C, Li H, Xiong W, Pan S, Ren D, Dang B, Yang Y, Wu M, Liu L
Cell metabolism 2023 Sep 5;35(9):1563-1579.e8

LncRNA CCAT1 facilitates the progression of gastric cancer via PTBP1-mediated glycolysis enhancement.

Zhang C, Wang H, Liu Q, Dai S, Tian G, Wei X, Li X, Zhao L, Shan B
Journal of experimental & clinical cancer research : CR 2023 Sep 23;42(1):246

Pyruvate kinase M2 regulates mitochondrial homeostasis in cisplatin-induced acute kidney injury.

Xie W, He Q, Zhang Y, Xu X, Wen P, Cao H, Zhou Y, Luo J, Yang J, Jiang L
Cell death & disease 2023 Oct 10;14(10):663

Nuclear translocation of PKM2 mediates keratinocyte metabolic reprogramming in psoriasis.

Yang L, Zhang J, Hu C, Chen X, Yang Y, Tang H, Ding X, Yan Y
Experimental dermatology 2023 Nov;32(11):1960-1970

The survivin-ran inhibitor LLP-3 decreases oxidative phosphorylation, glycolysis and growth of neuroblastoma cells.

Galiger C, Zohora FT, Dorneburg C, Tews D, Debatin KM, Beltinger C
BMC cancer 2023 Nov 25;23(1):1148

Development of Novel Small-Molecule Activators of Pyruvate Kinase Muscle Isozyme 2, PKM2, to Reduce Photoreceptor Apoptosis.

Wubben TJ, Chaudhury S, Watch BT, Stuckey JA, Weh E, Fernando R, Goswami M, Pawar M, Rech JC, Besirli CG
Pharmaceuticals (Basel, Switzerland) 2023 May 6;16(5)

Curcumin inhibits liquid-liquid phase separation of fused in sarcoma and attenuates the sequestration of pyruvate kinase to restore cellular metabolism.

Shi WP, Lin WJ, Ge WY, Chen LL, Zhang TD, Guo WH, Liu JL, Yin DC
Food & function 2023 May 22;14(10):4621-4631

Dihydrotanshinone I preconditions myocardium against ischemic injury via PKM2 glutathionylation sensitive to ROS.

Wu X, Liu L, Zheng Q, Ye H, Yang H, Hao H, Li P
Acta pharmaceutica Sinica. B 2023 Jan;13(1):113-127

Protein kinase C epsilon promotes de novo lipogenesis and tumor growth in prostate cancer cells by regulating the phosphorylation and nuclear translocation of pyruvate kinase isoform M2.

Lai X, Liang Y, Jin J, Zhang H, Wu Z, Li G, Wang J, Zhang Z, Chen H, Zeng F, Deng F
Experimental cell research 2023 Jan 1;422(1):113427

Exosomal lncRNA HOTAIR induces PDL1(+) B cells to impede anti-tumor immunity in colorectal cancer.

Xie Z, Xia J, Jiao M, Zhao P, Wang Z, Lin S, Xing Y, Li Y, Lu Z, Zhong Z, Miao C, Zhou P, Qian J, Wang L, Zhang D, Gu J, Chu Y, Liu R
Biochemical and biophysical research communications 2023 Feb 12;644:112-121

Anti-tumor effects of novel alkannin derivatives with potent selectivity on comprehensive analysis.

Dai ZQ, Gao F, Zhang ZJ, Lu MJ, Luo YJ, Zhang T, Shang BX, Gu YH, Zeng Q, Gao S, Guo ZQ, Xu B, Lei HM
Phytomedicine : international journal of phytotherapy and phytopharmacology 2023 Aug;117:154912

Salvianolic acid A regulates pyroptosis of endothelial cells via directly targeting PKM2 and ameliorates diabetic atherosclerosis.

Zhu J, Chen H, Le Y, Guo J, Liu Z, Dou X, Lu D
Frontiers in pharmacology 2022;13:1009229

The MBNL1/circNTRK2/PAX5 pathway regulates aerobic glycolysis in glioblastoma cells by encoding a novel protein NTRK2-243aa.

Zhao Y, Song J, Dong W, Liu X, Yang C, Wang D, Xue Y, Ruan X, Liu L, Wang P, Zhang M, Liu Y
Cell death & disease 2022 Sep 5;13(9):767

Aurora A-mediated pyruvate kinase M2 phosphorylation promotes biosynthesis with glycolytic metabolites and tumor cell cycle progression.

Jiang Y, Wang T, Sheng D, Han C, Xu T, Zhang P, You W, Fan W, Zhang Z, Jin T, Duan X, Yuan X, Liu X, Zhang K, Ruan K, Shi J, Guo J, Cheng A, Yang Z
The Journal of biological chemistry 2022 Nov;298(11):102561

Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues.

Fan TW, Daneshmandi S, Cassel TA, Uddin MB, Sledziona J, Thompson PT, Lin P, Higashi RM, Lane AN
Journal of immunology (Baltimore, Md. : 1950) 2022 Nov 1;209(9):1674-1690

Pyruvate Kinase M2 Protects Heart from Pressure Overload-Induced Heart Failure by Phosphorylating RAC1.

Ni L, Lin B, Hu L, Zhang R, Fu F, Shen M, Yang J, Shi D
Journal of the American Heart Association 2022 Jun 7;11(11):e024854

Development of a one-plasmid system to replace the endogenous protein with point mutation for post-translational modification studies.

Ishrat I, Cheng A, Yu F, Guo J, Zhang P, Zhang K, Yang Z
Molecular biology reports 2022 Jan;49(1):1-7

The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.

Zhu Y, Jin L, Shi R, Li J, Wang Y, Zhang L, Liang CZ, Narayana VK, De Souza DP, Thorne RF, Zhang LR, Zhang XD, Wu M
Molecular cell 2022 Feb 3;82(3):542-554.e6

Tetrahydroxy Stilbene Glucoside Alleviates Ischemic Stroke by Regulating Conformation-Dependent Intracellular Distribution of PKM2 for M2 Macrophage Polarization.

Li M, Lu W, Meng Y, Zhang W, Wang F, Sun L, Xu Y
Journal of agricultural and food chemistry 2022 Dec 14;70(49):15449-15463

Chemoproteomics reveals berberine directly binds to PKM2 to inhibit the progression of colorectal cancer.

Yan SH, Hu LM, Hao XH, Liu J, Tan XY, Geng ZR, Ma J, Wang ZL
iScience 2022 Aug 19;25(8):104773

Study on the Antitumor Effect and Glycolysis of Andrographolide in Anaplastic Thyroid Carcinoma.

Yang K, Wu K, Feng J, Yutian L, Zhu X, Xu D
Evidence-based complementary and alternative medicine : eCAM 2021;2021:5526581

Qian Yang Yu Yin Granule Improves Renal Injury of Hypertension by Regulating Metabolic Reprogramming Mediated by HIF-1α/PKM2 Positive Feedback Loop.

Qian L, Ren S, Xu Z, Zheng Y, Wu L, Yang Y, Wang Y, Li J, Yan S, Fang Z
Frontiers in pharmacology 2021;12:667433

Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.

Wu X, Liu L, Zheng Q, Hao H, Ye H, Li P, Yang H
Acta pharmaceutica Sinica. B 2021 Nov;11(11):3553-3566

Rapamycin facilitates differentiation of regulatory T cells via enhancement of oxidative phosphorylation.

Chen X, Li S, Long D, Shan J, Li Y
Cellular immunology 2021 Jul;365:104378

Shikonin differentially regulates glucose metabolism via PKM2 and HIF1α to overcome apoptosis in a refractory HCC cell line.

Yang W, Liu J, Hou L, Chen Q, Liu Y
Life sciences 2021 Jan 15;265:118796

Rab25 acts as an oncogene and participates in the regulation of aerobic glycolysis via PKM2 in gastric adenocarcinoma.

Zheng T, Huang L, Chen N, Xue H, Ni P, Huang G
Translational cancer research 2021 Feb;10(2):790-805

Pyruvate kinase M2 (PKM2) interacts with activating transcription factor 2 (ATF2) to bridge glycolysis and pyroptosis in microglia.

Li M, Lu H, Wang X, Duan C, Zhu X, Zhang Y, Ge X, Ji F, Wang X, Su J, Zhang D
Molecular immunology 2021 Dec;140:250-266

Endoplasmic reticulum stress promotes sorafenib resistance via miR-188-5p/hnRNPA2B1-mediated upregulation of PKM2 in hepatocellular carcinoma.

Zhou B, Lu D, Wang A, Cui J, Zhang L, Li J, Fan L, Wei W, Liu J, Sun G
Molecular therapy. Nucleic acids 2021 Dec 3;26:1051-1065

Dihydroartemisinin Inhibits the Proliferation of Leukemia Cells K562 by Suppressing PKM2 and GLUT1 Mediated Aerobic Glycolysis.

Gao P, Shen S, Li X, Liu D, Meng Y, Liu Y, Zhu Y, Zhang J, Luo P, Gu L
Drug design, development and therapy 2020;14:2091-2100

Danqi Pill Protects Against Heart Failure Post-Acute Myocardial Infarction via HIF-1α/PGC-1α Mediated Glucose Metabolism Pathway.

Zhang Q, Guo D, Wang Y, Wang X, Wang Q, Wu Y, Li C, Wang W, Wang Y
Frontiers in pharmacology 2020;11:458

SQSTM1-dependent autophagic degradation of PKM2 inhibits the production of mature IL1B/IL-1β and contributes to LIPUS-mediated anti-inflammatory effect.

Zhang B, Chen H, Ouyang J, Xie Y, Chen L, Tan Q, Du X, Su N, Ni Z, Chen L
Autophagy 2020 Jul;16(7):1262-1278

Dietary fish oil supplement induces age-specific contractile and proteomic responses in muscles of male rats.

Russ DW, Dimova K, Morris E, Pacheco M, Garvey SM, Scordilis SP
Lipids in health and disease 2020 Jul 9;19(1):165

Simvastatin re-sensitizes hepatocellular carcinoma cells to sorafenib by inhibiting HIF-1α/PPAR-γ/PKM2-mediated glycolysis.

Feng J, Dai W, Mao Y, Wu L, Li J, Chen K, Yu Q, Kong R, Li S, Zhang J, Ji J, Wu J, Mo W, Xu X, Guo C
Journal of experimental & clinical cancer research : CR 2020 Jan 30;39(1):24

Supra-coronary aortic banding improves right ventricular function in experimental pulmonary arterial hypertension in rats by increasing systolic right coronary artery perfusion.

Tian L, Xiong PY, Alizadeh E, Lima PDA, Potus F, Mewburn J, Martin A, Chen KH, Archer SL
Acta physiologica (Oxford, England) 2020 Aug;229(4):e13483

Regulation of the pyruvate metabolism node by monogene and polygene engineering of HEK-293 cells.

Xie L, Miao J, Li X, Yi X, Chu J
RSC advances 2019 Oct 31;9(61):35760-35770

PKM2 is the target of proanthocyanidin B2 during the inhibition of hepatocellular carcinoma.

Feng J, Wu L, Ji J, Chen K, Yu Q, Zhang J, Chen J, Mao Y, Wang F, Dai W, Xu L, Wu J, Guo C
Journal of experimental & clinical cancer research : CR 2019 May 17;38(1):204

miR-122 inhibits cancer cell malignancy by targeting PKM2 in gallbladder carcinoma.

Lu W, Zhang Y, Zhou L, Wang X, Mu J, Jiang L, Hu Y, Dong P, Liu Y
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2015 Nov 6;

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Western blot analysis of PK-M2-specific in various tissues and cell lines using Proteintech antibody 60268-1-Ig at dilution of 1:1000
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The PK-M2-specific antibody from Proteintech is a mouse monoclonal antibody to a peptide of human PK-M2-specific. This antibody recognizes human,mouse, rat antigen. The PK-M2-specific antibody has been validated for the following applications: ELISA, IHC, IF, WB analysis.
Appendix: 60268-1-Ig.pdf

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human colon cancer using 60268-1-Ig(PK-M2-specific antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human colon cancer using 60268-1-Ig(PK-M2-specific antibody) at dilution of 1:50 (under 40x lens)
Sample type: tissue