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LearnAb111970
antibody from Aladdin Scientific
Targeting: KEAP1
INrf2, KIAA0132, KLHL19, MGC10630, MGC1114, MGC20887, MGC4407, MGC9454
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- Ab111970 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant Keap1 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human Keap1 Antibody, Recombinant, could be used for WB, IHC and so on.Application:WB: 1/500 - 1/1000IHC : 1/50 - 1/100 Protein FunctionRetains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome. Post-translationalUbiquitinated and subject to proteasomal degradation.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 321-609- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,9 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Keap1 Antibody (Ab111970) - Western Blot All lanes: Recombinant Keap1 Antibody (Ab111970) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 70 kDa Observed band size: 64 kDa Exposure time: 10.0 s
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Keap1 Antibody (Ab111970) - Western Blot All lanes: Recombinant Keap1 Antibody (Ab111970) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 70 kDa Observed band size: 64 kDa Exposure time: 20.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Keap1 Antibody (Ab111970) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant Keap1 Antibody (Ab111970). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
