PA5-28368

antibody from Invitrogen Antibodies

Targeting: KEAP1 INrf2, KIAA0132, KLHL19, MGC10630, MGC1114, MGC20887, MGC4407, MGC9454

Antibody data

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Validation data

KEAP1 protein structure - PA5-28368 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of KEAP1 using 30 µg of NT2D1 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a KEAP1 polyclonal antibody (Product # PA5-28368) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-KEAP1 Polyclonal Antibody (Product # PA5-28368) and a 69.6 kDa band corresponding to KEAP1 was observed across the cell lines except for MCF7, DU 145 and A549 which are reported to have low to negative expression for KEAP1 (10.1371/journal.pone.0135257. eCollection 2015). An uncharacterized band (*) were also observed in some of the cell models tested. Whole cell extracts (1%SDS) (30 µg lysate) of HEL 92.1.7 (Lane 1), MCF7 (Lane 2), MDA-MB-231 (Lane 3), K-562 (Lane 4), PANC-1 (Lane 5), DU 145 (Lane 6) and A549 (Lane 7) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of KEAP1 was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a KEAP1 Polyclonal Antibody (Product # PA5-28368) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.