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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Other assay [1]
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- Product number
- 720288 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BDKRB2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Heteromerization fingerprints between bradykinin B2 and thromboxane TP receptors in native cells.
Dagher OK, Jaffa MA, Habib A, Ziyadeh FN, Jaffa AA
PloS one 2019;14(5):e0216908
PloS one 2019;14(5):e0216908
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 5 B2R-TP interactions in RASMC as revealed by co-IP followed by SDS PAGE. RASMC lysates were immunoprecipitated with anti-B2R followed by immunoblotting with anti-TP (upper panels) and anti-B2R (lower panels) antibodies, successively. E and L represent eluates and matching lysates of unstimulated RASMC (E1, L1), or RASMC that were stimulated with BK 10-11 M (E2, L2), IBOP 10-7 M (E3, L3), or [BK 10-11 M + IBOP 10-7 M] (E4, L4) for 10 min. EM and LM represent eluates and matching lysates of mock co-IP condition. (EB) represents eluates of a control co-IP condition, whereby Dynabeads-protein-A-anti-B2R were incubated with PBS instead of RASMC lysates. Images are representative of three qualitatively similar independent experiments. A denatured broad molecular weight protein ladder was loaded in parallel (upper and lower left-hand lanes).
