720275

antibody from Invitrogen Antibodies

Targeting: GRM2 GPRC1B, mGlu2, MGLUR2

Western blot

Antibody data

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Validation data

Product number: 720275 - Provider product page
Provider: Invitrogen Antibodies
Product name: mGluR2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature. This antibody is predicted to react with Monkey, Rabbit and Bovine.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 0.5 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

GRM2 protein structure - 720275 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HEK 293 cells were grown on poly-L-lysine-coated coverslips overnight. Cells were then transiently transfected with mGLUR2. After 24 h, cells were fixed with 4% paraformaldehyde and 0.2% picric acid in phosphate buffer (pH 6.9) for 30 min at room temperature and washed several times with phosphate buffer. Specimens were permeabilized with ice-cold methanol and then incubated with Anti-mGLUR2 Rabbit Polyclonal Antibody (Product # 720275 at a concentration of 2 µg/mL). Cells were then incubated with Alexa488-conjugated secondary antibody for 2 h at room temperature, mounted and examined using a laser scanning confocal microscope. Note, strong staining of transfected cells and no staining of adjacent non-transfected cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HEK 293 cells were grown on poly-L-lysine-coated coverslips overnight. Cells were then transiently transfected with mGLUR2. After 24 h, cells were fixed with 4% paraformaldehyde and 0.2% picric acid in phosphate buffer (pH 6.9) for 30 min at room temperature and washed several times with phosphate buffer. Specimens were permeabilized with ice-cold methanol and then incubated with Anti-mGLUR2 Rabbit Polyclonal Antibody (Product # 720275 at a concentration of 2 µg/mL). Cells were then incubated with Alexa488-conjugated secondary antibody for 2 h at room temperature, mounted and examined using a laser scanning confocal microscope. Note, strong staining of transfected cells and no staining of adjacent non-transfected cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HEK 293 cells were grown on poly-L-lysine-coated coverslips overnight. Cells were then transiently transfected with mGLUR2. After 24 h, cells were fixed with 4% paraformaldehyde and 0.2% picric acid in phosphate buffer (pH 6.9) for 30 min at room temperature and washed several times with phosphate buffer. Specimens were permeabilized with ice-cold methanol and then incubated with Anti-mGLUR2 Rabbit Polyclonal Antibody (Product # 720275 at a concentration of 2 µg/mL). Cells were then incubated with Alexa488-conjugated secondary antibody for 2 h at room temperature, mounted and examined using a laser scanning confocal microscope. Note, strong staining of transfected cells and no staining of adjacent non-transfected cells.