702592

antibody from Invitrogen Antibodies

Targeting: GRM2 GPRC1B, mGlu2, MGLUR2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

702592

Provider

Invitrogen Antibodies

Product name

mGLuR2 Recombinant Rabbit Monoclonal Antibody (15H9L5)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with Monkey, Rabbit, Bovine. Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

15H9L5

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on HEK-293 cells stably transfected with human mGLUR2 (Lane 2) and Mock (Lane 1). Receptors were eluted from the beads using SDS sample buffer for 20 min at 45 °C. Extracts were separated on 7.5% SDS-polyacrylamide gels and blotted onto PVDF membranes. The blot was probed with Anti-mGLuR2 Recombinant Rabbit Monoclonal Antibody (Product # 702592, 1 µg/mL, 1:500 dilution). A 120 kDa band and a 240 kDa band corresponding to the monomeric and dimeric forms of mGLuR2 was observed in the transfected cell line.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

For immunofluorescence analysis, HEK-293 cells were stably transfected with human mGLuR2. After 24hr of transfection the cells were fixed and permeabilized for detection of endogenous mGLuR2 using Anti-mGLuR2 Recombinant Rabbit Monoclonal Antibody (Product # 702592, 1 µg/mL, 1:500 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate. Panel a) shows representative cells that were stained for detection of membrane localization of mGLuR2 on the transfected cells. The images were captured at 40X magnification.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

For immunofluorescence analysis, HEK-293 cells were stably transfected with human mGLuR2. After 24hr of transfection the cells were fixed and permeabilized for detection of endogenous mGLuR2 using Anti-mGLuR2 Recombinant Rabbit Monoclonal Antibody (Product # 702592, 1 µg/mL, 1:500 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate. Panel a) shows representative cells that were stained for detection of membrane localization of mGLuR2 on the transfected cells. The images were captured at 40X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sections of Rat Brain, Rat Cerebellum and Human Brain were dewaxed, microwaved in citric acid and incubated with Anti-mGLuR2 Recombinant Rabbit Monoclonal Antibody (Product # 702592, 1 µg/mL, 1:500 dilution). Sections were then sequentially treated with biotinylated Anti-rabbit IgG and AB solution. Sections were further developed in AEC and lightly counterstained with hematoxylin. Panel a, b & c) shows the specific localization of mGLuR2 in neuronal stomata and dendrites in Rat Brain, Rat Cerebellum and Human Brain respectively. Panel d) shows the no primary antibody control.