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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- F54694 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CCR4-NOT transcription complex subunit 4 Antibody / CNOT4
- Antibody type
- Polyclonal
- Description
- This highly specific CCR4-NOT transcription complex subunit 4 antibody is suitable for use in Western blot/Flow cytometry/Immunohistochemistry applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the CCR4-NOT transcription complex subunit 4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) MCF7, 2) K562 and 3) HEK293 cell lysate with CCR4-NOT transcription complex subunit 4 antibody. Expected molecular weight: 48-84 kDa (multiple isoforms).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of mouse cerebellum tissue lysate with CCR4-NOT transcription complex subunit 4 antibody. Expected molecular weight: 48-84 kDa (multiple isoforms).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human testis tissue with CCR4-NOT transcription complex subunit 4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human U-2 OS cells with CCR4-NOT transcription complex subunit 4 antibody; Blue=isotype control, Green= CCR4-NOT transcription complex subunit 4 antibody.
