H00007321-M01

antibody from Abnova Corporation

Targeting: UBE2D1 E2(17)KB1, SFT, UBC4/5, UBCH5, UbcH5A

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007321-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007321-M01, RRID:AB_607263

Product name

UBE2D1 monoclonal antibody (M01), clone 2C6

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant UBE2D1.

Antigen sequence

MALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQA
TIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKIAFT
TKIYHPNINSNGSICLDILRSQWS


Isotype

IgG

Antibody clone number

2C6

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

The stability of herpes simplex virus 1 ICP0 early after infection is defined by the RING finger and the UL13 protein kinase.

Zhu Z, Du T, Zhou G, Roizman B
Journal of virology 2014 May;88(10):5437-43

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of UBE2D1 expression in transfected 293T cell line by UBE2D1 monoclonal antibody (M01), clone 2C6.Lane 1: UBE2D1 transfected lysate(16.6 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged UBE2D1 is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to UBE2D1 on formalin-fixed paraffin-embedded human cervix cancer. [antibody concentration 1 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol