Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- ABIN393588 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1) (AA 1-95) antibody
- Antibody type
- Monoclonal
- Description
- This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Mouse
- Epitope
- AA 1-95
- Isotype
- IgG
- Antibody clone number
- 2C6
- Vial size
- 100 μg
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Submitted references Priming and extending: a UbcH5/Cdc34 E2 handoff mechanism for polyubiquitination on a SCF substrate.
Insights into the conformational dynamics of the E3 ubiquitin ligase CHIP in complex with chaperones and E2 enzymes.
A comprehensive framework of E2-RING E3 interactions of the human ubiquitin-proteasome system.
Analysis of the human E2 ubiquitin conjugating enzyme protein interaction network.
Key role of Ubc5 and lysine-63 polyubiquitination in viral activation of IRF3.
Wu K, Kovacev J, Pan ZQ
Molecular cell 2010 Mar 26;37(6):784-96
Molecular cell 2010 Mar 26;37(6):784-96
Insights into the conformational dynamics of the E3 ubiquitin ligase CHIP in complex with chaperones and E2 enzymes.
Graf C, Stankiewicz M, Nikolay R, Mayer MP
Biochemistry 2010 Mar 16;49(10):2121-9
Biochemistry 2010 Mar 16;49(10):2121-9
A comprehensive framework of E2-RING E3 interactions of the human ubiquitin-proteasome system.
van Wijk SJ, de Vries SJ, Kemmeren P, Huang A, Boelens R, Bonvin AM, Timmers HT
Molecular systems biology 2009;5:295
Molecular systems biology 2009;5:295
Analysis of the human E2 ubiquitin conjugating enzyme protein interaction network.
Markson G, Kiel C, Hyde R, Brown S, Charalabous P, Bremm A, Semple J, Woodsmith J, Duley S, Salehi-Ashtiani K, Vidal M, Komander D, Serrano L, Lehner P, Sanderson CM
Genome research 2009 Oct;19(10):1905-11
Genome research 2009 Oct;19(10):1905-11
Key role of Ubc5 and lysine-63 polyubiquitination in viral activation of IRF3.
Zeng W, Xu M, Liu S, Sun L, Chen ZJ
Molecular cell 2009 Oct 23;36(2):315-25
Molecular cell 2009 Oct 23;36(2):315-25
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Supportive validation
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- WB
- Submitted by
- antibodies-online (provider)
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- Experimental details
- WB
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- IHC
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- IHC