100-401-931

antibody from Invitrogen Antibodies

Targeting: CCNT2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

100-401-931

Provider

Invitrogen Antibodies

Product name

Cyclin T2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

85 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot using Rocklands Anti-Cyclin T2 (Rabbit) is shown to detect two major bands (arrowheads) corresponding to human Cyclin T2a (~74kDa) and T2b (~81kDa) as indicated. Approximately 33 µg of a HeLa whole cell lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking the membrane with 5% BLOTTO (p/n B501-0500) in PBS, the membrane was probed for overnight at 4° C with the primary antibody diluted to 1:500 in 5% BLOTTO in PBS. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (611-132-122) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (700 nm channel, red). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.