PAB29449

antibody from Abnova Corporation

Targeting: LIMA1 EPLIN

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29449

Provider

Abnova Corporation

Product name

LIMA1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human LIMA1.

Antigen sequence

RPHKDLWASKNENEEILERPAQLANARETPHSPGV
EDAPIAKVGVLAASMEAKASSQQEKEDKPAETKKL
RIAWPPPTELGSSGSALEEGI


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Human cell line RT-4 with LIMA1 polyclonal antibody (Cat # PAB29449) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with LIMA1 polyclonal antibody (Cat # PAB29449) at 1-4 ug/mL concentration shows positivity in plasma membrane, actin filaments and focal adhesion sites.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney with LIMA1 polyclonal antibody (Cat # PAB29449) shows strong membranous positivity in cells in tubules at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)