Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-62659 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EPLIN Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: RPHKDLWASK NENEEILERP AQLANARETP HSPGVEDAPI AKVGVLAASM EAKASSQQEK EDKPAETKKL RIAWPPPTEL GSSGSALEEG I Highest antigen sequence identity to the following orthologs: Mouse - 85%, Rat - 82%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EPLin in human cell lines A-431 and A-549 using a EPLIN Polyclonal Antibody (Product # PA5-62659). Corresponding LIMA1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EPLIN using EPLIN Polyclonal Antibody (Product # PA5-62659) (A) shows similar pattern to an independent EPLIN Polyclonal Antibody (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of EPLIN in human cell line U-2 OS shows positivity in plasma membrane, actin filaments & focal adhesion sites. Samples were probed using an EPLIN Polyclonal Antibody (Product # PA5-62659).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of EPLIN in human liver using EPLIN Polyclonal Antibody (Product # PA5-62659).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of EPLIN in human testis using EPLIN Polyclonal Antibody (Product # PA5-62659).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of EPLIN in human rectum and pancreas tissues using EPLIN Polyclonal Antibody (Product # PA5-62659). Corresponding LIMA1 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of EPLIN in human liver, pancreas, rectum and testis using EPLIN Polyclonal Antibody (Product # PA5-62659) (A) shows similar protein distribution across tissues to an independent EPLIN Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of EPLIN in human pancreas using EPLIN Polyclonal Antibody (Product # PA5-62659) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of EPLIN in human rectum using EPLIN Polyclonal Antibody (Product # PA5-62659) shows high expression.